| http://purl.uniprot.org/citations/17289662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17289662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17289662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/17289662 | http://www.w3.org/2000/01/rdf-schema#comment | "In plants, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase (mitHPPK/DHPS) is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. Mining the Arabidopsis genome data base has revealed a second gene encoding a protein that lacks a potential transit peptide, suggesting a cytosolic localization of the isoenzyme (cytHPPK/DHPS). When the N-terminal part of the cytHPPK/DHPS was fused to green fluorescent protein, the fusion protein appeared only in the cytosol, confirming the above prediction. Functionality of cytHPPK/DHPS was addressed by two parallel approaches: first, the cytHPPK/DHPS was able to rescue yeast mutants lacking corresponding activities; second, recombinant cytHPPK/DHPS expressed and purified from Escherichia coli displayed both HPPK and DHPS activities in vitro. In contrast to mitHPPK/DHPS, which was ubiquitously expressed, the cytHPPK/DHPS gene was exclusively expressed in reproductive tissue, more precisely in developing seeds as revealed by histochemical analysis of a transgenic cytHPPK/DHPS promoter-GUS line. In addition, it was observed that expression of cytHPPK/DHPS mRNA was induced by salt stress, suggesting a potential role of the enzyme in stress response. This was supported by the phenotype of a T-DNA insertion mutant in the cytHPPK/DHPS gene, resulting in lower germination rates as compared with the wild-type upon application of oxidative and osmotic stress."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m701158200"xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m701158200"xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Ravanel S."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Ravanel S."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Storozhenko S."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Storozhenko S."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Van Der Straeten D."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Van Der Straeten D."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Zhang G.F."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Zhang G.F."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Rebeille F."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Rebeille F."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Bastien O."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Bastien O."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Lambert W."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Lambert W."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "De Brouwer V."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "De Brouwer V."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Chaerle P."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Chaerle P."xsd:string |
| http://purl.uniprot.org/citations/17289662 | http://purl.uniprot.org/core/author | "Navarrete O."xsd:string |