| http://purl.uniprot.org/citations/17293613 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17293613 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17293613 | http://www.w3.org/2000/01/rdf-schema#comment | "Group IVA cytosolic phospholipase A(2) (cPLA(2)alpha) initiates eicosanoid production; however, this pathway is not completely ablated in cPLA(2)alpha(-/-) lung fibroblasts stimulated with A23187 or serum. cPLA(2)alpha(+/+) fibroblasts preferentially released arachidonic acid, but A23187-stimulated cPLA(2)alpha(-/-) fibroblasts nonspecifically released multiple fatty acids. Arachidonic acid release from cPLA(2) alpha(-/-) fibroblasts was inhibited by the cPLA(2)alpha inhibitors pyrrolidine-2 (IC(50), 0.03 microM) and Wyeth-1 (IC(50), 0.1 microM), implicating another C2 domain-containing group IV PLA(2). cPLA(2) alpha(-/-) fibroblasts contain cPLA(2)beta and cPLA(2)zeta but not cPLA(2)epsilon or cPLA(2)delta. Purified cPLA(2)zeta exhibited much higher lysophospholipase and PLA(2) activity than cPLA(2)beta and was potently inhibited by pyrrolidine-2 and Wyeth-1, which did not inhibit cPLA(2)beta. In contrast to cPLA(2)beta, cPLA(2)zeta expressed in Sf9 cells mediated A23187-induced arachidonic acid release, which was inhibited by pyrrolidine-2 and Wyeth-1. cPLA(2)zeta exhibits specific activity, inhibitor sensitivity, and low micromolar calcium dependence similar to cPLA(2)alpha and has been identified as the PLA(2) responsible for calcium-induced fatty acid release and prostaglandin E(2) production from cPLA(2) alpha(-/-) lung fibroblasts. In response to ionomycin, EGFP-cPLA(2)zeta translocated to ruffles and dynamic vesicular structures, whereas EGFP-cPLA(2)alpha translocated to the Golgi and endoplasmic reticulum, suggesting distinct mechanisms of regulation for the two enzymes."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m608458200"xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m608458200"xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M608458200"xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Gelb M.H."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Gelb M.H."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Ghosh M."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Ghosh M."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Ghomashchi F."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Ghomashchi F."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Bonventre J.V."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Bonventre J.V."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Leslie C.C."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Leslie C.C."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Loper R."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Loper R."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Tucker D.E."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/author | "Tucker D.E."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17293613 | http://purl.uniprot.org/core/pages | "11676-11686"xsd:string |