| http://purl.uniprot.org/citations/17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17314511 | http://www.w3.org/2000/01/rdf-schema#comment | "The c-MYC oncogene encodes a transcription factor, which is sufficient and necessary for the induction of cellular proliferation. However, the c-MYC protein is a relatively weak transactivator suggesting that it may have other functions. To identify protein interactors which may reveal new functions or represent regulators of c-MYC we systematically identified proteins associated with c-MYC in vivo using a proteomic approach. We combined tandem affinity purification (TAP) with the mass spectral multidimensional protein identification technology (MudPIT). Thereby, 221 c-MYC-associated proteins were identified. Among them were 17 previously known c-MYC-interactors. Selected new c-MYC-associated proteins (DBC-1, FBX29, KU70, MCM7, Mi2-beta/CHD4, RNA Pol II, RFC2, RFC3, SV40 Large T Antigen, TCP1alpha, U5-116kD, ZNF281) were confirmed independently. For association with MCM7, SV40 Large T Antigen and DBC-1 the functionally important MYC-box II region was required, whereas FBX29 and Mi2-beta interacted via MYC-box II and the BR-HLH-LZ motif. In addition, regulators of c-MYC activity were identified: ectopic expression of FBX29, an E3 ubiquitin ligase, decreased c-MYC protein levels and inhibited c-MYC transactivation, whereas knock-down of FBX29 elevated the concentration of c-MYC. Furthermore, sucrose gradient analysis demonstrated that c-MYC is present in numerous complexes with varying size and composition, which may accommodate the large number of new c-MYC-associated proteins identified here and mediate the diverse functions of c-MYC. Our results suggest that c-MYC, besides acting as a mitogenic transcription factor, regulates cellular proliferation by direct association with protein complexes involved in multiple synthetic processes required for cell division, as for example DNA-replication/repair and RNA-processing. Furthermore, this first comprehensive description of the c-MYC-associated sub-proteome will facilitate further studies aimed to elucidate the biology of c-MYC."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.org/dc/terms/identifier | "doi:10.4161/cc.6.2.3742"xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Hermeking H."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Zhang R."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Yates J.R."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Bailey A."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Menssen A."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Koch H.B."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Zhang C.D."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/author | "Verdoodt B."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/name | "Cell Cycle"xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/pages | "205-217"xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/title | "Large-scale identification of c-MYC-associated proteins using a combined TAP/MudPIT approach."xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/17314511 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17314511 |
| http://purl.uniprot.org/citations/17314511 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17314511 |
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| http://purl.uniprot.org/uniprot/#_P52701-mappedCitation-17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17314511 |
| http://purl.uniprot.org/uniprot/#_P53007-mappedCitation-17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17314511 |
| http://purl.uniprot.org/uniprot/#_P61619-mappedCitation-17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17314511 |
| http://purl.uniprot.org/uniprot/#_Q07864-mappedCitation-17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17314511 |
| http://purl.uniprot.org/uniprot/#_Q14204-mappedCitation-17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17314511 |
| http://purl.uniprot.org/uniprot/#_Q15029-mappedCitation-17314511 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17314511 |