| http://purl.uniprot.org/citations/17315274 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17315274 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17315274 | http://www.w3.org/2000/01/rdf-schema#comment | "Variation in the snake venom proteome is well documented and it is a ubiquitous phenomenon at all taxonomical levels. However, variation in the snake venom peptidome is so far not described. In this work we used mass spectrometry [liquid chromatography/mass spectrometry (LC/MS) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOFMS)] to explore sex-based differences among the venom peptides of eighteen sibling specimens of Bothrops jararaca of a single litter born and raised in the laboratory. MALDI-TOFMS analyses showed individual variability among the bradykinin-potentiating peptides (BPPs), and, interestingly, four new peptides were detected only in female venoms and identified by de novo sequencing as cleaved BPPs lacking the C-terminal Q-I-P-P sequence. Similar results were obtained with venom from wild-caught adult non-sibling specimens of B. jararaca and in this case we were able to identify the gender of the specimen by analyzing the MALDI-TOF profile of the peptide fraction and finding the cleaved peptides only in female venoms. Synthetic replicates of the cleaved BPPs were less potent than the full-length BPP-10c in potentiating the bradykinin hypotensive effect, suggesting that the C-terminus is critical for the interaction of the BPPs with their mammalian molecular targets. This work represents a comprehensive mass spectrometric analysis of the peptide fraction of B. jararaca venom and shows for the first time sex-based differences in the snake venom peptidome of sibling and non-sibling snakes and suggests that the BPPs may follow distinct processing pathways in female and male individuals."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.org/dc/terms/identifier | "doi:10.1002/rcm.2931"xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.org/dc/terms/identifier | "doi:10.1002/rcm.2931"xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Pimenta D.C."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Pimenta D.C."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Konno K."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Konno K."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Furtado M.F."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Furtado M.F."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Prezoto B.C."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Prezoto B.C."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Serrano S.M.T."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "Serrano S.M.T."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "de Camargo A.C.M."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "de Camargo A.C.M."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "de Melo R.L."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/author | "de Melo R.L."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/name | "Rapid Commun. Mass Spectrom."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/name | "Rapid Commun. Mass Spectrom."xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/pages | "1034-1042"xsd:string |
| http://purl.uniprot.org/citations/17315274 | http://purl.uniprot.org/core/pages | "1034-1042"xsd:string |