| http://purl.uniprot.org/citations/17322287 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17322287 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17322287 | http://www.w3.org/2000/01/rdf-schema#comment | "The availability of an annotated genome sequence for the yeast Saccharomyces cerevisiae has made possible the proteome-scale study of protein function and protein-protein interactions. These studies rely on availability of cloned open reading frame (ORF) collections that can be used for cell-free or cell-based protein expression. Several yeast ORF collections are available, but their use and data interpretation can be hindered by reliance on now out-of-date annotations, the inflexible presence of N- or C-terminal tags, and/or the unknown presence of mutations introduced during the cloning process. High-throughput biochemical and genetic analyses would benefit from a "gold standard" (fully sequence-verified, high-quality) ORF collection, which allows for high confidence in and reproducibility of experimental results. Here, we describe Yeast FLEXGene, a S. cerevisiae protein-coding clone collection that covers over 5000 predicted protein-coding sequences. The clone set covers 87% of the current S. cerevisiae genome annotation and includes full sequencing of each ORF insert. Availability of this collection makes possible a wide variety of studies from purified proteins to mutation suppression analysis, which should contribute to a global understanding of yeast protein function."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.6037607"xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.6037607"xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Camargo A.A."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Camargo A.A."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Hu Y."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Hu Y."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Moreira D."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Moreira D."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "LaBaer J."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "LaBaer J."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Simpson A.J.G."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Simpson A.J.G."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Raphael J."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Raphael J."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Rolfs A."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Rolfs A."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Zuo D."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Zuo D."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Williamson J."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Williamson J."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |
| http://purl.uniprot.org/citations/17322287 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |