| http://purl.uniprot.org/citations/17327229 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17327229 | http://www.w3.org/2000/01/rdf-schema#comment | "N-MYC is a transcription factor that plays an important role in cellular survival in neuroblastoma, and amplification of the N-MYC oncogene is the primary adverse prognostic indicator for neuroblastoma. Focal adhesion kinase (FAK) is a survival factor that has been shown to be overexpressed in many types of human cancers. In this study, we investigated the role of N-MYC regulation of FAK expression in neuroblastoma. We first found a correlation between N-MYC and FAK expression in neuroblastoma. Real time quantitative PCR demonstrated an increase in FAK mRNA abundance in the N-MYC-amplified IMR-32 compared with the nonamplified SK-N-AS neuroblastoma cell lines. FAK protein expression also correlated positively with N-MYC expression in the N-MYC-amplified IMR-32 versus nonamplified SK-N-AS neuroblastoma cell lines. The same results were seen with the isogenic N-MYC(+) (Tet(-)) and N-MYC(-) (Tet(+)) neuroblastoma cell lines. Promoter-reporter assays showed that activity of the FAK promoter was increased in the N-MYC-amplified IMR-32 cell line, in the N-MYC-transfected SK-N-AS nonamplified cell line, and in the isogenic N-MYC(+) (Tet(-)) neuroblastoma cell lines compared with the nonamplified and N-MYC-nonexpressing cell lines. We also identified two N-MYC binding sites in the FAK promoter sequence and showed binding of N-MYC transcription factor to the FAK promoter through electrophoretic mobility shift, chromatin immunoprecipitation, and dual luciferase assays. Finally down-regulation of FAK expression in N-MYC-inducible neuroblastoma cell lines with FAK small interfering RNA or a dominant-negative FAK inhibitor (AdFAK-CD) significantly decreased viability and increased apoptosis in the N-MYC(+) (Tet(-)) cells compared with the isogenic N-MYC(-) (Tet(+)) cells, demonstrating the biological significance of FAK overexpression in the N-MYC-expressing cell lines. This is the first report linking N-MYC and FAK in neuroblastoma, and it clearly demonstrates that N-MYC induces FAK expression. The results indicate that N-MYC regulation of FAK expression can control cellular functions in isogenic N-MYC(-/+) (Tet(+/-)) neuroblastoma cell lines."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m701450200"xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Ma X."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Finch R."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Cance W.G."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Golubovskaya V.M."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Kurenova E.V."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Trujillo A."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Beierle E.A."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Nagaram A."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/author | "Vella J."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/pages | "12503-12516"xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/title | "N-MYC regulates focal adhesion kinase expression in human neuroblastoma."xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17327229 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17327229 |
| http://purl.uniprot.org/citations/17327229 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17327229 |
| http://purl.uniprot.org/uniprot/P04198#attribution-6DFA295D1D09ACFBF619CE4FBFB33896 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17327229 |
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| http://purl.uniprot.org/uniprot/#_B4DRZ1-mappedCitation-17327229 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17327229 |