| http://purl.uniprot.org/citations/17329398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17329398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17329398 | http://www.w3.org/2000/01/rdf-schema#comment | "We have identified the presence of leupaxin (LPXN), which belongs to the paxillin extended family of focal adhesion-associated adaptor proteins, in prostate cancer cells. Previous studies have demonstrated that LPXN is a component of the podosomal signaling complex found in osteoclasts, where LPXN was found to associate with the protein tyrosine kinases Pyk2 and c-Src and the cytosolic protein tyrosine phosphatase-proline-, glutamate-, serine-, and threonine-rich sequence (PTP-PEST). In the current study, LPXN was detectable as a 50-kDa protein in PC-3 cells, a bone-derived metastatic prostate cancer cell line. In PC-3 cells, LPXN was also found to associate with Pyk2, c-Src, and PTP-PEST. A siRNA-mediated inhibition of LPXN resulted in decreased in vitro PC-3 cell migration. A recombinant adenoviral-mediated overexpression of LPXN resulted in an increased association of Pyk2 with LPXN, whereas a similar adenoviral-mediated overexpression of PTP-PEST resulted in decreased association of Pyk2 and c-Src with LPXN. The overexpression of LPXN in PC-3 cells resulted in increased migration, as assessed by in vitro Transwell migration assays. On the contrary, the overexpression of PTP-PEST in PC-3 cells resulted in decreased migration. The overexpression of LPXN resulted in increased activity of Rho GTPase, which was decreased in PTP-PEST-overexpressing cells. The increase in Rho GTPase activity following overexpression of LPXN was inhibited in the presence of Y27632, a selective inhibitor of Rho GTPase. In conclusion, our data demonstrate that LPXN forms a signaling complex with Pyk2, c-Src, and PTP-PEST to regulate migration of prostate cancer cells."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpcell.00503.2006"xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpcell.00503.2006"xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Gupta A."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Gupta A."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Bai G."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Bai G."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Nunez S."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Nunez S."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Sahu S.N."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/author | "Sahu S.N."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/pages | "C2288-C2296"xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/pages | "C2288-C2296"xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/title | "Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/title | "Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells."xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/volume | "292"xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://purl.uniprot.org/core/volume | "292"xsd:string |
| http://purl.uniprot.org/citations/17329398 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17329398 |
| http://purl.uniprot.org/citations/17329398 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17329398 |