| http://purl.uniprot.org/citations/17332742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17332742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17332742 | http://www.w3.org/2000/01/rdf-schema#comment | "Little is known about the higher-order structure of prespliceosomal A complexes, in which pairing of the pre-mRNA's splice sites occurs. Here, human A complexes were isolated under physiological conditions by double-affinity selection. Purified complexes contained stoichiometric amounts of U1, U2 and pre-mRNA, and crosslinking studies indicated that these form concomitant base pairing interactions with one another. A complexes contained nearly all U1 and U2 proteins plus approximately 50 non-snRNP proteins. Unexpectedly, proteins of the hPrp19/CDC5 complex were also detected, even when A complexes were formed in the absence of U4/U6 snRNPs, demonstrating that they associate independent of the tri-snRNP. Double-affinity purification yielded structurally homogeneous A complexes as evidenced by electron microscopy, and allowed for the first time the generation of a three-dimensional structure. A complexes possess an asymmetric shape (approximately 260 x 200 x 195 angstroms) and contain a main body with various protruding elements, including a head-like domain and foot-like protrusions. Complexes isolated here are well suited for in vitro assembly studies to determine factor requirements for the A to B complex transition."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7601631"xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.emboj.7601631"xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Stark H."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Stark H."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Urlaub H."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Urlaub H."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Kastner B."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Kastner B."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Dube P."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Dube P."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Sander B."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Sander B."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Golas M.M."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Golas M.M."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Hartmuth K."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Hartmuth K."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Will C.L."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Will C.L."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Deckert J."xsd:string |
| http://purl.uniprot.org/citations/17332742 | http://purl.uniprot.org/core/author | "Deckert J."xsd:string |