| http://purl.uniprot.org/citations/17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17346885 | http://www.w3.org/2000/01/rdf-schema#comment | "The present study was undertaken to examine the effect of amitriptyline on the antinociceptive effect of morphine and its underlying mechanisms in regulating glutamate transporters trafficking in morphine-tolerant rats. Long-term morphine infusion induced antinociceptive tolerance and down-regulation of glutamate transporters (GTs), GLAST, GLT-1, and EAAC1, expression in the rat spinal cord dorsal horn. Acute amitriptyline treatment potentiated morphine's antinociceptive effect, with a 5.3-fold leftward shift of morphine's dose-response curve in morphine-tolerant rats, and this was associated with GLAST and GLT-1 trafficking onto the cell surface. Similar to our previous studies, morphine challenge (10 microg/10 microl, i.t.) significant by increased the excitatory amino acids (EAAs) aspartate and glutamate level in the CSF dialysates of morphine-tolerant rats. Acute amitriptyline treatment not only suppressed this morphine-evoked EAA release, but further reduced the EAA concentration than baseline level. Furthermore, long-term morphine infusion up-regulated PKA and PKC protein expression in the spinal cord dorsal horn, while amitriptyline inhibited the increase in expression of phospho-PKA, PKCalpha, PKCbetaII, and PKCgamma. In morphine-tolerant rats, acute treatment with PKA inhibitor H89 and PKC inhibitor Gö6805 attenuated morphine tolerance and the morphine-induced CSF glutamate and aspartate elevation, and induced trafficking of GLAST and GLT-1 from cytosol onto the cell surface. These results show that acute amitriptyline treatment preserved morphine's antinociceptive effect in morphine-tolerant rats; the mechanisms may be involved in inhibition of phospho-PKA and PKC expression, and thus inducing the GLAST and GLT-1 trafficking onto glial cell surface which enhances the EAA uptake from the synaptic cleft and reduces EAA concentration in the spinal CSF."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pain.2007.01.031"xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Wang Y.C."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Wang J.J."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Wang Y.H."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Liu T.M."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Wong C.S."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Tai Y.H."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Tsai R.Y."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/author | "Tao P.L."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/name | "Pain"xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/pages | "343-354"xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/title | "Amitriptyline preserves morphine's antinociceptive effect by regulating the glutamate transporter GLAST and GLT-1 trafficking and excitatory amino acids concentration in morphine-tolerant rats."xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://purl.uniprot.org/core/volume | "129"xsd:string |
| http://purl.uniprot.org/citations/17346885 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17346885 |
| http://purl.uniprot.org/citations/17346885 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17346885 |
| http://purl.uniprot.org/uniprot/#_A0A0G2K611-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |
| http://purl.uniprot.org/uniprot/#_A0A0G2KAS7-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JSU1-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |
| http://purl.uniprot.org/uniprot/#_A0A159KIH4-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |
| http://purl.uniprot.org/uniprot/#_A0A159KIL2-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |
| http://purl.uniprot.org/uniprot/#_A6HNP2-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |
| http://purl.uniprot.org/uniprot/#_A6HNP3-mappedCitation-17346885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17346885 |