| http://purl.uniprot.org/citations/17360967 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17360967 | http://www.w3.org/2000/01/rdf-schema#comment | "The clathrin adaptors AP-1 and AP-2 bind cargo proteins via two types of motifs: tyrosine-based Yxx phi and dileucine-based [DE]XXXL[LI]. Although it is well established that Yxx phi motifs bind to the mu subunits of AP-1 or AP-2, dileucine motifs have been reported to bind to either the mu or beta subunits of these adaptors as well as the gamma/sigma1 hemicomplex of AP-1. To clarify this controversy, the various subunits of AP-1 and AP-2 were expressed individually and in hemicomplex form in insect cells, and they were used in glutathione S-transferase pull-down assays to determine their binding properties. We report that the gamma/sigma1 or alpha/sigma2 hemicomplexes bound the dileucine-based motifs of several proteins quite strongly, whereas binding by the beta1/mu1 and beta2/mu2 hemicomplexes, and the individual beta or mu subunits, was extremely weak or undetectable. The gamma/sigma1 and alpha/sigma2 hemicomplexes displayed substantial differences in their preference for particular dileucine-based motifs. Most strikingly, an aspartate at position -4 compromised binding to the gamma/sigma1 hemicomplex, whereas minimally affecting binding to alpha/sigma2. There was an excellent correlation between binding to the alpha/sigma2 hemicomplex and in vivo internalization mediated by the dileucine-based sorting signals. These findings provide new insights into the trafficking mechanisms of D/EXXXL[LI]-mediated sorting signals."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e07-01-0012"xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/author | "Lee I."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/author | "Kornfeld S."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/author | "Bu G."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/author | "Doray B."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/author | "Knisely J."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/pages | "1887-1896"xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/title | "The gamma/sigma1 and alpha/sigma2 hemicomplexes of clathrin adaptors AP-1 and AP-2 harbor the dileucine recognition site."xsd:string |
| http://purl.uniprot.org/citations/17360967 | http://purl.uniprot.org/core/volume | "18"xsd:string |
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