| http://purl.uniprot.org/citations/17407764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17407764 | http://www.w3.org/2000/01/rdf-schema#comment | "Hsp70 chaperones are composed of two domains; the 40 kDa N-terminal nucleotide-binding domain (NDB) and the 30 kDa C-terminal substrate-binding domain (SBD). Structures of the SBD from Escherichia coli homologues DnaK and HscA show it can be further divided into an 18 kDa beta-sandwich subdomain, which forms the hydrophobic binding pocket, and a 10 kDa C-terminal three-helix bundle that forms a lid over the binding pocket. Across prokaryotes and eukaryotes, the NBD and beta-sandwich subdomain are well conserved in both sequence and structure. The C-terminal subdomain is, however, more evolutionary variable and the only eukaryotic structure from rat Hsc70 revealed a diverged helix-loop-helix fold. We have solved the crystal structure of the C-terminal 10 kDa subdomain from Caenorhabditis elegans Hsp70 which forms a helical-bundle similar to the prokaryotic homologues. This provides the first confirmation of the structural conservation of this subdomain in eukaryotes. Comparison with the rat structure reveals a domain-swap dimerisation mechanism; however, the C. elegans subdomain exists exclusively as a monomer in solution in agreement with the hypothesis that regions out with the C-terminal subdomain are necessary for Hsp70 self-association."xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2007.03.107"xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/author | "Walkinshaw M.D."xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/author | "Worrall L.J."xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/pages | "105-110"xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/title | "Crystal structure of the C-terminal three-helix bundle subdomain of C. elegans Hsp70."xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://purl.uniprot.org/core/volume | "357"xsd:string |
| http://purl.uniprot.org/citations/17407764 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17407764 |
| http://purl.uniprot.org/citations/17407764 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17407764 |
| http://purl.uniprot.org/uniprot/#_P09446-mappedCitation-17407764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17407764 |
| http://purl.uniprot.org/uniprot/P09446 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17407764 |