| http://purl.uniprot.org/citations/17445765 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17445765 | http://www.w3.org/2000/01/rdf-schema#comment | "Homeodomain repressor factor Hesx1/Rpx plays a crucial role in the formation of Rathke's pouch at the start of pituitary organogenesis and represses the Prop-1-dependent expression of Pit-1 gene, which promotes the differentiation of Pit-1-dependent hormone producing cells. Recently, we discovered a novel function of Prop-1 by which it activates the porcine follicle stimulating hormone beta subunit (FSHbeta) gene through Fd2 region (-852/-746). The present study aimed to determine whether Hesx1 exerts its role in the Prop-1-dependent activation of FSHbeta gene. Transient transfection assay for the porcine FSHbeta promoter -985/+10, electrophoretic mobility shift assay (EMSA) and DNase I footprinting analysis for Fd2 region were carried out. Transfection assay in GH3 cells demonstrated that expression of Hesx1 alone does not change the promoter activity but the coexpression with Prop-1 represses the Prop-1-dependent activation of FSHbeta promoter. Similar results were obtained for the mutant reporter vector deleting the region -745/-104 indicating that Fd2 region is a target site of Hesx1 as well as Prop-1. EMSA and DNase I footprinting analysis using recombinant Hesx1 and Prop-1 protein demonstrated that Hesx1 and Prop-1 certainly bind to the AT-rich regions in a different manner. These results suggest that Hesx1 blocks the advanced expression of FSHbeta gene in the early stage of pituitary development, and Prop-1 thereafter appears and activates this gene."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2007.03.197"xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/author | "Kato Y."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/author | "Kato T."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/author | "Nakayama M."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/author | "Kitahara K."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/author | "Susa T."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/author | "Kimoto F."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/pages | "712-717"xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/title | "Homeodomain transcription factor Hesx1/Rpx occupies Prop-1 activation sites in porcine follicle stimulating hormone (FSH) beta subunit promoter."xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://purl.uniprot.org/core/volume | "357"xsd:string |
| http://purl.uniprot.org/citations/17445765 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17445765 |
| http://purl.uniprot.org/citations/17445765 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17445765 |
| http://purl.uniprot.org/uniprot/Q61658#attribution-A0D896A9F59526C0EFED14C4B15F806D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17445765 |
| http://purl.uniprot.org/uniprot/Q61658#attribution-F4E7B9CB9B1A342C45A2FD8F3AEC307D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17445765 |
| http://purl.uniprot.org/uniprot/#_Q61658-mappedCitation-17445765 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17445765 |
| http://purl.uniprot.org/uniprot/Q61658 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17445765 |