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http://purl.uniprot.org/citations/17455230http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17455230http://www.w3.org/2000/01/rdf-schema#comment"

Background

The crucial role of CD4 T-cells in anti-tumor immune response is widely recognized, yet the identification of HLA class II-restricted epitopes derived from tumor antigens has lagged behind compared to class I epitopes. This is particularly true for prostate cancer. Based on the hypothesis that successful cancer immunotherapy will likely resemble autoimmunity, we searched for the CD4 T-cell epitopes derived from prostatic proteins that are restricted by human leukocyte antigen (HLA)-DRB1*1501, an allele associated with granulomatous prostatitis (GP), a disease that may have an autoimmune etiology. One of the antigens implicated in the development of autoimmunity in the prostate is prostatic acid phosphatase (PAP), which is also considered a promising target for prostate cancer immunotherapy.

Methods

We immunized transgenic (tg) mice engineered to express HLA-DRB1*1501 with human PAP. A library of overlapping 20-mer peptides spanning the entire human PAP sequence was screened in vitro for T-cell recognition by proliferative and interferon (IFN)-gamma enzyme-linked immunosorbent spot (ELISPOT) assays.

Results

We identified two 20-mer peptides, PAP (133-152), and PAP (173-192), that were immunogenic and naturally processed from whole PAP in HLA-DRB1*1501 tg mice. These peptides were also capable of stimulating CD4 T lymphocytes from HLA-DRB1*1501-positive patients with GP and normal donors.

Conclusions

These peptides can be used for the design of a new generation of peptide-based vaccines against prostate cancer. The study can also be helpful in understanding the role of autoimmunity in the development of some forms of chronic prostatitis."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.org/dc/terms/identifier"doi:10.1002/pros.20575"xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/author"Vandenbark A.A."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/author"Kouiavskaia D.V."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/author"Alexander R.B."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/author"Klyushnenkova E.N."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/author"Kodak J.A."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/name"Prostate"xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/pages"1019-1028"xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/title"Identification of HLA-DRB1*1501-restricted T-cell epitopes from human prostatic acid phosphatase."xsd:string
http://purl.uniprot.org/citations/17455230http://purl.uniprot.org/core/volume"67"xsd:string
http://purl.uniprot.org/citations/17455230http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17455230
http://purl.uniprot.org/citations/17455230http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17455230
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http://purl.uniprot.org/uniprot/#_A0A0E3DD60-mappedCitation-17455230http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17455230
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