| http://purl.uniprot.org/citations/17456548 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17456548 | http://www.w3.org/2000/01/rdf-schema#comment | "Meiotic DNA double strand breaks (DSBs) are indicated at leptotene by the phosphorylated form of histone H2AX (gamma-H2AX). In contrast to previous studies, we identified on both zygotene and pachytene chromosomes two distinct types of gamma-H2AX foci: multiple small (S) foci located along autosomal synaptonemal complexes (SCs) and larger signals on chromatin loops (L-foci). The S-foci number gradually declined throughout pachytene, in parallel with the repair of DSBs monitored by repair proteins suggesting that S-foci mark DSB repair events. We validated this interpretation by showing the absence of S-foci in Spo11(-/-) spermatocytes. By contrast, the L-foci number was very low through pachytene. Based on the analysis of gamma-H2AX labeling after irradiation of spermatocytes, the formation of DSBs clearly induced L-foci formation. Upon DSB repair, these foci appear to be processed and lead to the above mentioned S-foci. The presence of L-foci in wild-type pachytene and diplotene could therefore reflect delayed or unregulated DSB repair events. Interestingly, their distribution was different in Spo11(+/-) spermatocytes compared with Spo11(+/+) spermatocytes, where DSB repair might be differently regulated as a response to homeostatic control of crossing-over. The presence of these L-foci in Spo11(-/-) spermatocytes raises the interesting possibility of yet uncharacterized alterations in DNA or chromosome structure in Spo11(-/-) cells."xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.004945"xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/author | "de Massy B."xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/author | "Dutrillaux B."xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/author | "Bernardino-Sgherri J."xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/author | "Chicheportiche A."xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/pages | "1733-1742"xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/title | "Characterization of Spo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse."xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://purl.uniprot.org/core/volume | "120"xsd:string |
| http://purl.uniprot.org/citations/17456548 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17456548 |
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| http://purl.uniprot.org/uniprot/#_Q9WTK8-mappedCitation-17456548 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17456548 |
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