| http://purl.uniprot.org/citations/1747371 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1747371 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1747371 | http://www.w3.org/2000/01/rdf-schema#comment | "A new protein, called alboaggregin-B (AL-B), has been isolated from Trimeresurus albolabris venom by ion-exchange chromatography. It agglutinated platelets without the need for Ca2+ or any other cofactor. The purified protein showed an apparent molecular mass on SDS-PAGE and gel filtration of about 23 kDa under nonreducing conditions. Ristocetin did not alter the binding of AL-B to platelets or affect AL-B-induced platelet agglutination. Agglutinating activity was not dependent on either proteolytic or lectin-like activity in AL-B. Binding analysis showed that AL-B bound to platelets with high affinity (Kd = 13.6 +/-9.3 nM) at approximately 30,800 +/-14,300 binding sites per platelet. AL-B inhibited the binding of labeled bovine von Willebrand factor (vWF) to platelets. Monoclonal antibodies against the 45-kDa N-terminal domain of platelet glycoprotein Ib inhibited the binding both of AL-B and of bovine vWF to platelets, and also inhibited platelet agglutination induced by AL-B and bovine vWF. Specific removal of the N-terminal domain of GPIb by treatment of the platelets with elastase or Serratia marcescens protease reduced the binding of labeled AL-B and bovine vWF to platelets and blocked platelet agglutination caused by both agonists. Monoclonal antibodies to glycoprotein IIb/IIIa, to bovine vWF, and to bovine serum albumin did not show any effect on the binding of AL-B to platelets. Our results indicate that the binding domain for AL-B on platelet GPIb is close to or identical with the one for vWF. This new protein may be a very useful tool for studying the interaction between platelets and vWF."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00113a007"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00113a007"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/author | "Lu W."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/author | "Lu W."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/author | "Peng M."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/author | "Peng M."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/author | "Kirby E.P."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/author | "Kirby E.P."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/pages | "11529-11536"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/pages | "11529-11536"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/title | "Alboaggregin-B: a new platelet agonist that binds to platelet membrane glycoprotein Ib."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/title | "Alboaggregin-B: a new platelet agonist that binds to platelet membrane glycoprotein Ib."xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/1747371 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1747371 |
| http://purl.uniprot.org/citations/1747371 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1747371 |
| http://purl.uniprot.org/citations/1747371 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1747371 |
| http://purl.uniprot.org/citations/1747371 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1747371 |