| http://purl.uniprot.org/citations/17488829 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17488829 | http://www.w3.org/2000/01/rdf-schema#comment | "Vesicular transport during exocytosis is regulated by Rab GTPase (Sec4p in yeast), which is activated by a guanine nucleotide exchange factor (GEF) called Sec2p. Here, we report the crystal structure of the Sec2p GEF domain in a complex with the nucleotide-free Sec4p at 2.7 A resolution. Upon complex formation, the Sec2p helices approach each other, flipping the side chain of Phe-109 toward Leu-104 and Leu-108 of Sec2p. These three residues provide a hydrophobic platform to attract the side chains of Phe-49, Ile-53, and Ile-55 in the switch I region as well as Phe-57 and Trp-74 in the interswitch region of Sec4p. Consequently, the switch I and II regions are largely deformed, to create a flat hydrophobic interface that snugly fits the surface of the Sec2p coiled coil. These drastic conformational changes disrupt the interactions between switch I and the bound guanine nucleotide, which facilitates the GDP release. Unlike the recently reported 3.3 A structure of the Sec4p.Sec2p complex, our structure contains a phosphate ion bound to the P-loop, which may represent an intermediate state of the nucleotide exchange reaction."xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0701550104"xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/author | "Sato Y."xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/author | "Ishitani R."xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/author | "Nureki O."xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/author | "Fukai S."xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/pages | "8305-8310"xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/title | "Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state."xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://purl.uniprot.org/core/volume | "104"xsd:string |
| http://purl.uniprot.org/citations/17488829 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17488829 |
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