| http://purl.uniprot.org/citations/17492382 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17492382 | http://www.w3.org/2000/01/rdf-schema#comment | "Continuous-flow analysis, where samples circulate encapsulated in a carrier fluid is an attractive alternative to batch processing for high-throughput devices that use the polymerase chain reaction (PCR). Challenges of continuous-flow prototypes include the hydrodynamic and biological incompatibility of the carrier fluid, microchannel fouling, sample carryover and the integration of a nucleic acid extraction and reverse transcription step. We tested two homemade, continuous-flow thermocycler microdevices for amplification of reverse-transcribed messages from cell lysates without nucleic acid extraction. Amplification yield and specificity were assessed with state-of-the-art, real-time quantitative equipment. Carryover contamination between consecutive samples was absent. Amplification specificity and interference by genomic DNA were optimized by primer design. Robust detection of the low-copy transcript CLIC5 from 18 cells per microliter is demonstrated in cultured lymphoblasts. The results prove the concept that the development of micro-total analysis systems (micro-TAS) for continuous gene expression directly from cell suspensions is viable with current technology."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10544-007-9083-1"xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/author | "Gonzalez A."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/author | "Davies M."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/author | "Ciobanu D."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/author | "Dalton T."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/author | "Sayers M."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/author | "Sirr N."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/name | "Biomed Microdevices"xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/pages | "729-736"xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/title | "Gene transcript amplification from cell lysates in continuous-flow microfluidic devices."xsd:string |
| http://purl.uniprot.org/citations/17492382 | http://purl.uniprot.org/core/volume | "9"xsd:string |
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