| http://purl.uniprot.org/citations/17511964 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17511964 | http://www.w3.org/2000/01/rdf-schema#comment | "2-DE related techniques have been broadly used for protein separation and identification in recent years. However, many important cellular components often escape detection by 2-DE due to its inherent low resolution. Here we use a polyU-affinity column to enrich proteins with nucleotide-binding motifs. As a result, 26 enriched protein spots, including three new Arabidopsis RNA-binding proteins cp31A, cp31B, and CSP41, are identified by MALDI-TOF-MS. In the sum, 15 protein spots encoded by 12 individual genes are observed on the 2-DE only after binding to the polyU-affinity column. Two of these genes are expressed at lower than 0.1% the level of TUB4 that were considered mainly as non-expressed genes in several ATH1 microarray analysis of Arabidopsis leaf samples. Our results suggest that polyU or similar media may be combined with 2-DE techniques to identify cellular proteins with extremely low transcription levels to fulfill specific research needs."xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2007.04.189"xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/author | "Wang B.C."xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/author | "Zhu Y.X."xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/pages | "808-812"xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/title | "Identification of proteins expressed at extremely low level in Arabidopsis leaves."xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://purl.uniprot.org/core/volume | "358"xsd:string |
| http://purl.uniprot.org/citations/17511964 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17511964 |
| http://purl.uniprot.org/citations/17511964 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17511964 |
| http://purl.uniprot.org/uniprot/Q8RW96#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q9ZUU4#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q04836#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q9FGS0#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q9LYA9#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/P83484#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q9FKW6#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q8W493#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q9SZJ5#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q93ZC9#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/Q9C9W5#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |
| http://purl.uniprot.org/uniprot/P23321#attribution-9CDAC899EF5B0379B4058A5E7D97A013 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17511964 |