| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/2000/01/rdf-schema#comment | "In the ancient organisms, methanogenic archaea, lacking the canonical cysteinyl-tRNA synthetase, Cys-tRNA(Cys) is produced by an indirect pathway, in which O-phosphoseryl-tRNA synthetase ligates O-phosphoserine (Sep) to tRNA(Cys) and Sep-tRNA:Cys-tRNA synthase (SepCysS) converts Sep-tRNA(Cys) to Cys-tRNA(Cys). In this study, the crystal structure of SepCysS from Archaeoglobus fulgidus has been determined at 2.4 A resolution. SepCysS forms a dimer, composed of monomers bearing large and small domains. The large domain harbors the seven-stranded beta-sheet, which is typical of the pyridoxal 5'-phosphate (PLP)-dependent enzymes. In the active site, which is located near the dimer interface, PLP is covalently bound to the side-chain of the conserved Lys209. In the proximity of PLP, a sulfate ion is bound by the side-chains of the conserved Arg79, His103, and Tyr104 residues. The active site is located deep within the large, basic cleft to accommodate Sep-tRNA(Cys). On the basis of the surface electrostatic potential, the amino acid residue conservation mapping, the position of the bound sulfate ion, and the substrate amino acid binding manner in other PLP-dependent enzymes, a binding model of Sep-tRNA(Cys) to SepCysS was constructed. One of the three strictly conserved Cys residues (Cys39, Cys42, or Cys247), of one subunit may play a crucial role in the catalysis in the active site of the other subunit."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2007.04.050"xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2007.04.050"xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/author | "Yokoyama S."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/author | "Fukunaga R."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/author | "Fukunaga R."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/pages | "128-141"xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/pages | "128-141"xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/title | "Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/title | "Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus."xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/volume | "370"xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://purl.uniprot.org/core/volume | "370"xsd:string |
| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17512006 |
| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17512006 |
| http://purl.uniprot.org/citations/17512006 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17512006 |
| http://purl.uniprot.org/citations/17512006 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17512006 |
| http://purl.uniprot.org/citations/17512006 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17512006 |