http://purl.uniprot.org/citations/17517107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/17517107 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/17517107 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundSesame allergy is increasingly being reported, and multi-sensitization to peanut and tree nuts has been described. The clinical relevance and cross-reactivity of many sesame proteins, such as Ses i 6, are unknown.ObjectiveThe aims of this study were to perform a preliminary examination of the cross-reactivity of Ses i 6 in vitro, examine the ability of Ses i 6 to activate basophils in a modified basophil activation test (mBAT), and assess whether such an assay may help to distinguish between potentially relevant and irrelevant IgE reactivity towards 11S globulin proteins.MethodsInhibition immunoblotting and chicken anti-rJug r 4 antibodies were used to determine the cross-reactivity of rSes i 6. Basophils from atopic donors were stripped of resident IgE before passive sensitization with food-allergic sera and challenged with protein extracts or recombinant protein. Basophil activation was measured using two activation markers, CD203c and CD63, via flow cytometry.ResultsIgE immunoblotting showed cross-reactivity between rJug r 4 and rSes i 6 using sera from two human donors and chicken IgY. Additionally, rSes i 6 activated basophils passively sensitized with sesame-allergic sera. Cross-reactive serum from a sesame-allergic but walnut-tolerant donor was not able to activate basophils when challenged by walnut extract despite IgE reactivity to walnut determined by immunoblotting.ConclusionsThe sesame 11S globulin shows partial immunological cross-reactivity with walnut, and although it is classified as a minor allergen, activated basophils sensitized with serum from seven out of eleven sesame-allergic donors. Additionally, the mBAT may help distinguish between clinically relevant and irrelevant in vitro IgE cross-reactivity of seed storage proteins in nuts and seeds and thus warrants use in further studies."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2222.2007.02725.x"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2222.2007.02725.x"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Chen R.J."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Chen R.J."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Teuber S.S."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Teuber S.S."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Tzen J.T."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Tzen J.T."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Wallowitz M.L."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/author | "Wallowitz M.L."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/name | "Clin. Exp. Allergy"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/name | "Clin. Exp. Allergy"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/pages | "929-938"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/pages | "929-938"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/title | "Ses i 6, the sesame 11S globulin, can activate basophils and shows cross-reactivity with walnut in vitro."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/title | "Ses i 6, the sesame 11S globulin, can activate basophils and shows cross-reactivity with walnut in vitro."xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/volume | "37"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://purl.uniprot.org/core/volume | "37"xsd:string |
http://purl.uniprot.org/citations/17517107 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17517107 |
http://purl.uniprot.org/citations/17517107 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17517107 |