| http://purl.uniprot.org/citations/17517630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17517630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17517630 | http://www.w3.org/2000/01/rdf-schema#comment | "The three deleted in liver cancer genes (DLC1-3) encode Rho-GTPase-activating proteins (RhoGAPs) whose expression is frequently down-regulated or silenced in a variety of human malignancies. The RhoGAP activity is required for full DLC-dependent tumor suppressor activity. Here we report that DLC1 and DLC3 bind to human tensin1 and its chicken homolog. The binding has been mapped to the tensin Src homology 2 (SH2) and phosphotyrosine binding (PTB) domains at the C terminus of tensin proteins. Distinct DLC1 sequences are required for SH2 and PTB binding. DCL binding to both domains is constitutive under basal conditions. The SH2 binding depends on a tyrosine in DCL1 (Y442) but is phosphotyrosine-independent, a highly unusual feature for SH2 binding. DLC1 competed with the binding of other proteins to the tensin C terminus, including beta 3-integrin binding to the PTB domain. Point mutation of a critical tyrosine residue (Y442F) in DLC1 rendered the protein deficient for binding the tensin SH2 domain and binding full-length tensin. The Y442F protein was diffusely cytoplasmic, in contrast to the localization of wild-type DLC1 to focal adhesions, but it retained the ability to reduce the intracellular levels of Rho-GTP. The Y442F mutant displayed markedly reduced biological activity, as did a mutant that was RhoGAP-deficient. The results suggest that DLC1 is a multifunctional protein whose biological activity depends on cooperation between its tensin binding and RhoGAP activities, although neither activity depends on the other."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0703033104"xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0703033104"xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Li G."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Li G."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Qian X."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Qian X."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Popescu N.C."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Popescu N.C."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Yamada K.M."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Yamada K.M."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Vass W.C."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Vass W.C."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Lowy D.R."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Lowy D.R."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Papageorge A.G."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Papageorge A.G."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Asmussen H.K."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Asmussen H.K."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Asnaghi L."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Asnaghi L."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Braverman R."xsd:string |
| http://purl.uniprot.org/citations/17517630 | http://purl.uniprot.org/core/author | "Braverman R."xsd:string |