| http://purl.uniprot.org/citations/17518637 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17518637 | http://www.w3.org/2000/01/rdf-schema#comment | "Pluripotent embryonic stem cells (ESCs) have recently been considered as a primary material for regenerating tissues lost to injuries and degenerative diseases. For clinical implementation of this technology, a quality controlled, reproducible culture system is necessary for the expansion and differentiation of the cells. Used in many bioprocess applications, suspension bioreactors have gained considerable attention for the regulated large-scale expansion of cells. The current study presents a bioreactor process for the large-scale expansion of undifferentiated murine ESCs as aggregates. In this system, the level of ESC aggregation and differentiation was effectively controlled by adjusting shear forces and inoculation density, achieving a 31-fold expansion in 5 days. Pluripotency markers Oct-4, Nanog, SSEA-1, ALP, and rex-1 were assessed using flow cytometry analysis and gene expression profiles and showed that the undifferentiated nature of the cells within the ESC aggregates was maintained. Colony-forming efficiencies and embryoid body formation tests of the expanded cultures demonstrated that characteristic functional attributes of undifferentiated cells were not lost. Overcoming a major impediment in the area of ESC expansion, this study describes a successful process for the controlled and reproducible largescale expansion of ESCs using suspension culture bioreactors."xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.org/dc/terms/identifier | "doi:10.1089/ten.2006.12.3233"xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/author | "Rancourt D.E."xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/author | "zur Nieden N.I."xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/author | "Cormier J.T."xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/author | "Kallos M.S."xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/date | "2006"xsd:gYear |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/name | "Tissue Eng"xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/pages | "3233-3245"xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/title | "Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors."xsd:string |
| http://purl.uniprot.org/citations/17518637 | http://purl.uniprot.org/core/volume | "12"xsd:string |
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