| http://purl.uniprot.org/citations/17523149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17523149 | http://www.w3.org/2000/01/rdf-schema#comment | "Auxiliary beta-subunits associated with pore-forming Slo1 alpha-subunits play an essential role in regulating functional properties of large-conductance, voltage- and Ca(2+)-activated K(+) channels commonly termed BK channels. Even though both noninactivating and inactivating BK channels are thought to be regulated by beta-subunits (beta1, beta2, beta3, or beta4), the molecular determinants underlying inactivating BK channels in native cells have not been extensively demonstrated. In this study, rbeta2 (but not rbeta3-subunit) was identified as a molecular component in rat lumbar L4-6 dorsal root ganglia (DRG) by RT-PCR responsible for inactivating large-conductance Ca(2+)-dependent K(+) currents (BK(i) currents) in small sensory neurons. The properties of native BK(i) currents obtained from both whole-cell and inside-out patches are very similar to inactivating BK channels produced by co-expressing mSlo1 alpha- and hbeta2-subunits in Xenopus oocytes. Intracellular application of 0.5 mg/ml trypsin removes inactivation of BK(i) channels, and the specific blockers of BK channels, charybdotoxin (ChTX) and iberiotoxin (IbTX), inhibit these BK(i) currents. Single BK(i) channel currents derived from inside-out patches revealed that one BK(i) channel contained three rbeta2-subunits (on average), with a single-channel conductance about 217 pS under 160 K(+) symmetrical recording conditions. Blockade of BK(i) channels by 100 nM IbTX augmented firing frequency, broadened action potential waveform and reduced after-hyperpolarization. We propose that the BK(i) channels in small diameter DRG sensory neurons might play an important role in regulating nociceptive input to the central nervous system (CNS)."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.21007"xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Li W."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Wu Y."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Xu T."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Guo Z.H."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Ding J.P."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Gao S.B."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/author | "Lv C.X."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/pages | "348-357"xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/title | "Characterization of voltage-and Ca2+-activated K+ channels in rat dorsal root ganglion neurons."xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://purl.uniprot.org/core/volume | "212"xsd:string |
| http://purl.uniprot.org/citations/17523149 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17523149 |
| http://purl.uniprot.org/citations/17523149 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17523149 |
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