| http://purl.uniprot.org/citations/17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17548631 | http://www.w3.org/2000/01/rdf-schema#comment | "MHC class I molecules present peptides derived from the ectodomains of endogenous transmembrane proteins; however, the processing of these Ags is incompletely understood. As model transmembrane Ags we investigated the processing of MHC-I-derived fusion proteins containing the N-terminally extended K(b)-restricted OVA epitope SIINFEKL in the extracytoplasmic domain. In TAP-deficient, nonprofessional APCs, the epitope was cleaved out of various sequence contexts and presented to T cells. Ag presentation was inhibited by acidophilic amines and inhibitors of the vacuolar proton pump, indicating processing in endosomes. Endosomal aspartic-type cathepsins, and to some extent also the trans-Golgi network protease furin, were involved in processing. Clathrin-dependent and independent internalization from the cell surface targeted MHC-I fusion proteins to early and late endosomes, where SIINFEKL/K(b) complexes were detected by immunofluorescence microscopy. Targeting of MHC-I fusion proteins to processing compartments was independent of sequence motifs in the cytoplasmic tail. Not only TAP-deficient cells, but also TAP-competent APCs used the vacuolar pathway for processing of MHC-I fusion proteins. Thus, endosomal processing of internalized endogenous transmembrane proteins represents a novel alternate pathway for the generation of MHC-I-binding peptides."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.178.12.7932"xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/author | "Tiwari N."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/author | "Reinheckel T."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/author | "Moldenhauer G."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/author | "Momburg F."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/author | "Hammerling G.J."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/author | "Garbi N."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/pages | "7932-7942"xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/title | "A transporter associated with antigen-processing independent vacuolar pathway for the MHC class I-mediated presentation of endogenous transmembrane proteins."xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://purl.uniprot.org/core/volume | "178"xsd:string |
| http://purl.uniprot.org/citations/17548631 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17548631 |
| http://purl.uniprot.org/citations/17548631 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17548631 |
| http://purl.uniprot.org/uniprot/#_A0A068BER1-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_A0A068BIS1-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_A0A3Q4EGL0-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RQ98-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_A0A3Q4L2T5-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_D3Z401-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_D3YTP7-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_G3UZZ2-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |
| http://purl.uniprot.org/uniprot/#_F6QHF1-mappedCitation-17548631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17548631 |