| http://purl.uniprot.org/citations/17562857 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17562857 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17562857 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic mRNAs containing premature translation termination codons (PTCs) are rapidly degraded by a process termed "nonsense-mediated mRNA decay" (NMD). We examined protein-protein and protein-RNA interactions among Caenorhabditis elegans proteins required for NMD. SMG-2, SMG-3, and SMG-4 are orthologs of yeast (Saccharomyces cerevisiae) and mammalian Upf1, Upf2, and Upf3, respectively. A combination of immunoprecipitation and yeast two-hybrid experiments indicated that SMG-2 interacts with SMG-3, SMG-3 interacts with SMG-4, and SMG-2 interacts indirectly with SMG-4 via shared interactions with SMG-3. Such interactions are similar to those observed in yeast and mammalian cells. SMG-2-SMG-3-SMG-4 interactions require neither SMG-2 phosphorylation, which is abolished in smg-1 mutants, nor SMG-2 dephosphorylation, which is reduced or eliminated in smg-5 mutants. SMG-2 preferentially associates with PTC-containing mRNAs. We monitored the association of SMG-2, SMG-3, and SMG-4 with mRNAs of five endogenous genes whose mRNAs are alternatively spliced to either contain or not contain PTCs. SMG-2 associates with both PTC-free and PTC-containing mRNPs, but it strongly and preferentially associates with ("marks") those containing PTCs. SMG-2 marking of PTC-mRNPs is enhanced by SMG-3 and SMG-4, but SMG-3 and SMG-4 are not detectably associated with the same mRNPs. Neither SMG-2 phosphorylation nor dephosphorylation is required for selective association of SMG-2 with PTC-containing mRNPs, indicating that SMG-2 is phosphorylated only after premature terminations have been discriminated from normal terminations. We discuss these observations with regard to the functions of SMG-2 and its phosphorylation during NMD."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.org/dc/terms/identifier | "doi:10.1128/MCB.00410-07"xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.00410-07"xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Newman C.L."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Newman C.L."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Anderson P."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Anderson P."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Johns L."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Johns L."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Grimson A."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Grimson A."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Kuchma S.L."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/author | "Kuchma S.L."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/pages | "5630-5638"xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/pages | "5630-5638"xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/title | "Caenorhabditis elegans SMG-2 selectively marks mRNAs containing premature translation termination codons."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/title | "Caenorhabditis elegans SMG-2 selectively marks mRNAs containing premature translation termination codons."xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/17562857 | http://purl.uniprot.org/core/volume | "27"xsd:string |