http://purl.uniprot.org/citations/17565604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/17565604 | http://www.w3.org/2000/01/rdf-schema#comment | "The proprotein convertase PC1/3 preferentially cleaves its substrates in the dense core secretory granules of endocrine and neuroendocrine cells. Similar to most proteinases synthesized first as zymogens, PC1/3 is synthesized as a larger precursor that undergoes proteolytic processing of its signal peptide and propeptide. The N-terminally located propeptide has been shown to be essential for folding and self-inhibition. Furthermore, PC1/3 also possesses a C-terminal region (CT-peptide) which, for maximal enzymatic activity, must also be cleaved. To date, its role has been documented through transfection studies in terms of sorting and targeting of PC1/3 and chimeric proteins into secretory granules. In this study, we examined the properties of a 135-residue purified bacterially produced CT-peptide on the in vitro enzymatic activity of PC1/3. Depending on the amount of CT-peptide used, it is shown that the CT-peptide increases PC1/3 activity at low concentrations (nm) and decreases it at high concentrations (microm), a feature typical of an activator. Furthermore, we show that, contrary to the propeptide, the CT-peptide is not further cleaved by PC1/3 although it is sensitive to human furin activity. Based on these results, it is proposed that PC1/3, through its various domains, is capable of controlling its enzymatic activity in all regions of the cell that it encounters. This mode of self-control is unique among members of all proteinases families."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1742-4658.2007.05883.x"xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/author | "Lazure C."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/author | "Dikeakos J.D."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/author | "Gauthier D."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/author | "Rabah N."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/author | "Reudelhuber T.L."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/name | "FEBS J"xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/pages | "3482-3491"xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/title | "The C-terminal region of the proprotein convertase 1/3 (PC1/3) exerts a bimodal regulation of the enzyme activity in vitro."xsd:string |
http://purl.uniprot.org/citations/17565604 | http://purl.uniprot.org/core/volume | "274"xsd:string |
http://purl.uniprot.org/citations/17565604 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17565604 |
http://purl.uniprot.org/citations/17565604 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17565604 |
http://purl.uniprot.org/uniprot/#_Q32MU0-mappedCitation-17565604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17565604 |
http://purl.uniprot.org/uniprot/#_P63239-mappedCitation-17565604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17565604 |
http://purl.uniprot.org/uniprot/#_Q8BRQ2-mappedCitation-17565604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17565604 |
http://purl.uniprot.org/uniprot/Q8BRQ2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17565604 |
http://purl.uniprot.org/uniprot/P63239 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17565604 |
http://purl.uniprot.org/uniprot/Q32MU0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17565604 |