Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17573727http://www.w3.org/2000/01/rdf-schema#comment"

Background

Chromatin modification may play a role in inflammatory gene regulation in asthma. Cyclic adenosine mono-phosphate response element-binding protein (CREB), with the specific co-activator, the CREB-binding protein (CBP), contributes to the acetylation of chromatin and to the transcription of pro-inflammatory genes.

Objectives

To evaluate the expression of CBP and of phospho-CREB (p-CREB) in bronchial biopsies and in peripheral blood mononuclear cells (PBMC) of controls (C), untreated (UA), inhaled steroid treated (ICS) and steroid-dependent asthmatic (SDA) patients.

Methods

We used immunohistochemistry in bronchial biopsies and western blot analysis and immunocytochemistry in PBMC.

Results

Cyclic adenosine mono-phosphate response element-binding protein expression, in the epithelium was similar in all groups, while p-CREB expression was increased in UA and in SDA in comparison with ICS and C subjects (C vs UA P = 0.002, C vs SDA P = 0.007), (ICS vs SDA P = 0.005), (ICS vs UA P = 0.001). Interestingly, also in the submucosa, p-CREB was increased in UA and SDA in comparison with ICS and C subjects (C vs UA P = 0.0004) (C vs SDA P < 0.0001) (ICS vs UA P = 0.002) (ICS vs SDA P < 0.0001) and positively correlated with leukocyte infiltration within the bronchi (CD45RB+ cells). Similar results were obtained with PBMC isolated from the same patient groups. Incubation of PBMC in vitro, with fluticasone propionate, decreased the p-CREB expression induced by cytokine activation (interferon-gamma, tumor necrosis factor-alpha).

Conclusions

This study demonstrates that the expression of p-CREB is related, in asthma, to the persistent inflammation according to the disease severity. p-CREB expression can be modulated by glucocorticoids in responsive patients."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.org/dc/terms/identifier"doi:10.1111/j.1398-9995.2007.01417.x"xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Pompeo F."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Bousquet J."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Bruno A."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Pace E."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Chiappara G."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Bonsignore G."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Chanez P."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/author"Gjomarkaj M."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/name"Allergy"xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/pages"787-794"xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/title"Variable p-CREB expression depicts different asthma phenotypes."xsd:string
http://purl.uniprot.org/citations/17573727http://purl.uniprot.org/core/volume"62"xsd:string
http://purl.uniprot.org/citations/17573727http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17573727
http://purl.uniprot.org/citations/17573727http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17573727
http://purl.uniprot.org/uniprot/#_B7Z7P1-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
http://purl.uniprot.org/uniprot/#_B7Z5C6-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
http://purl.uniprot.org/uniprot/#_Q16118-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
http://purl.uniprot.org/uniprot/#_Q53X93-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
http://purl.uniprot.org/uniprot/#_P16220-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
http://purl.uniprot.org/uniprot/#_Q4ZG78-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
http://purl.uniprot.org/uniprot/#_Q53RU9-mappedCitation-17573727http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17573727
▶