| http://purl.uniprot.org/citations/17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17580303 | http://www.w3.org/2000/01/rdf-schema#comment | "Latent transforming growth factor-beta-binding proteins (LTBPs) are extracellular matrix (ECM) glycoproteins that play a major role in storage of latent TGF-beta in the ECM and regulate its availability. We have previously identified fibronectin as a key molecule for incorporation of LTBP1 and TGF-beta into the ECM of osteoblasts and fibroblasts. Here we provide evidence that heparan sulfate proteoglycans may mediate binding between LTBP1 and fibronectin. We have localized critical domains in the N terminus of LTBP1 that are required for co-localization with fibronectin in osteoblast cultures and have identified heparin binding sites in the N terminus of LTBP1 between residues 345 and 487. Solid-phase binding assays suggest that LTBP1 does not bind directly to fibronectin but that the binding is indirect. Heparin coupled to bovine serum albumin (heparin-BSA) was able to mediate binding between fibronectin and LTBP1. Treatment of primary osteoblast cultures with heparin or heparin-BSA but not with chondroitin sulfate impaired LTBP1 deposition onto fibronectin without inhibiting expression of LTBP1. Inhibition of LTBP1 incorporation was accompanied by reduced incorporation of latent TGF-beta into the ECM, with increased amounts of soluble latent TGF-beta. Inhibition of attachment of glycosaminoglycans to the core proteins of proteoglycans by beta-d-xylosides also reduced incorporation of LTBP1 into the ECM. These studies suggest that heparan sulfate proteoglycans may play a critical role in regulating TGF-beta availability by controlling the deposition of LTBP1 into the ECM in association with fibronectin."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m703341200"xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Chen Q."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Peters D.M."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Farach-Carson M.C."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Dallas S.L."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Sivakumar P."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Gomes R.R."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/author | "Barley C."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/pages | "26418-26430"xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/title | "Potential role for heparan sulfate proteoglycans in regulation of transforming growth factor-beta (TGF-beta) by modulating assembly of latent TGF-beta-binding protein-1."xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17580303 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17580303 |
| http://purl.uniprot.org/citations/17580303 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17580303 |
| http://purl.uniprot.org/uniprot/#_B4DST8-mappedCitation-17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/#_B4DRF7-mappedCitation-17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/#_B4DZD1-mappedCitation-17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/#_B4DNR7-mappedCitation-17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/#_B7ZLY3-mappedCitation-17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/#_Q14766-mappedCitation-17580303 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/Q14766 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17580303 |
| http://purl.uniprot.org/uniprot/B7ZLY3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17580303 |