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http://purl.uniprot.org/citations/1761038http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1761038http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1761038http://www.w3.org/2000/01/rdf-schema#comment"Sequence-specific 1H-NMR assignments are reported for the Tyr41----His (Y41H) mutant of the single-stranded DNA binding protein, encoded by gene V of the filamentous bacteriophage M13 (GVP). The mutant protein was chosen for this purpose because it exhibits significantly improved solubility characteristics over wild-type GVP [Folkers et al. (1991) Eur. J. Biochem. 200, 139-148]. The secondary structure elements present in the protein are deduced from a qualitative interpretation of the nuclear Overhauser enhancement spectra and amide exchange data. The protein is entirely composed of antiparallel beta-structure. It is shown that identical structural elements are present in wild-type GVP. Previously, we have demonstrated that the secondary structure of the beta-loop, encompassing residues 13-31 which is present in GVP in solution, deviates from that proposed for the same amino acid sequence on the basis of X-ray diffraction data [van Duynhoven et al. (1990) FEBS Lett. 261, 1-4]. Now that we have arrived at a complete description of the secondary structure of the protein in solution, other deviations with respect to the crystallographically determined structure became apparent as well. The N-terminal part of the protein is, in solution, part of a triple-stranded beta-sheet while, in the crystal, it is an extended strand pointing away from the bulk of the protein dimer. One of the antiparallel beta-sheets in the protein which had been designated earlier as the complex loop has, in the solution structure, a different pairwise arrangement of the residues in its respective beta-ladders. Residues 30 and 48 are opposite to one another in the solution structure while in the crystal structure residues 32 and 48 are paired. A similar observation is made for the so-called dyad domain of the protein of which the beta-sheet in the solution structure is shifted by one residue with respect to that of the crystal structure."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1991.tb16382.x"xsd:string
http://purl.uniprot.org/citations/1761038http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1991.tb16382.x"xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Konings R.N."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Konings R.N."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Folkers P.J.M."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Folkers P.J.M."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Harmsen B.J.M."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Harmsen B.J.M."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Jonker A.J."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"Jonker A.J."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"van Duynhoven J.P.M."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/author"van Duynhoven J.P.M."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/date"1991"xsd:gYear
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/date"1991"xsd:gYear
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/pages"349-360"xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/pages"349-360"xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/title"Sequence-specific 1H-NMR assignment and secondary structure of the Tyr-41-->His mutant of the single-stranded DNA binding protein, gene V protein, encoded by the filamentous bacteriophage M13."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/title"Sequence-specific 1H-NMR assignment and secondary structure of the Tyr-41-->His mutant of the single-stranded DNA binding protein, gene V protein, encoded by the filamentous bacteriophage M13."xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/volume"202"xsd:string
http://purl.uniprot.org/citations/1761038http://purl.uniprot.org/core/volume"202"xsd:string
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