| http://purl.uniprot.org/citations/17610847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17610847 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17610847 | http://www.w3.org/2000/01/rdf-schema#comment | "The omega-atracotoxins (omega-ACTX) are a family of arthropod-selective peptide neurotoxins from Australian funnel-web spider venoms (Hexathelidae: Atracinae) that are candidates for development as biopesticides. We isolated a 37-residue insect-selective neurotoxin, omega-ACTX-Ar1a, from the venom of the Sydney funnel-web spider Atrax robustus, with high homology to several previously characterized members of the omega-ACTX-1 family. The peptide induced potent excitatory symptoms, followed by flaccid paralysis leading to death, in acute toxicity tests in house crickets. Using isolated smooth and skeletal nerve-muscle preparations, the toxin was shown to lack overt vertebrate toxicity at concentrations up to 1 microM. To further characterize the target of the omega-ACTXs, voltage-clamp analysis using the whole-cell patch-clamp technique was undertaken using cockroach dorsal unpaired median neurons. It is shown here for the first time that omega-ACTX-Ar1a, and its homolog omega-ACTX-Hv1a from Hadronyche versuta, reversibly block both mid-low-(M-LVA) and high-voltage-activated (HVA) insect calcium channel (Ca(v)) currents. This block occurred in the absence of alterations in the voltage-dependence of Ca(v) channel activation, and was voltage-independent, suggesting that omega-ACTX-1 family toxins are pore blockers rather than gating modifiers. At a concentration of 1 microM omega-ACTX-Ar1a failed to significantly affect global K(v) channel currents. However, 1 microM omega-ACTX-Ar1a caused a modest 18% block of insect Na(v) channel currents, similar to the minor block of Na(v) channels reported for other insect Ca(v) channel blockers such as omega-agatoxin IVA. These findings validate both M-LVA and HVA Ca(v) channels as potential targets for insecticides."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2007.05.017"xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2007.05.017"xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Hains P.G."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Hains P.G."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Hodgson W.C."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Hodgson W.C."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "King G.F."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "King G.F."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Nicholson G.M."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Nicholson G.M."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Wen S."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Wen S."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Chong Y."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Chong Y."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Wilson D.T."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Wilson D.T."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Broady K.W."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Broady K.W."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Hayes J.L."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Hayes J.L."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Sollod B."xsd:string |
| http://purl.uniprot.org/citations/17610847 | http://purl.uniprot.org/core/author | "Sollod B."xsd:string |