| http://purl.uniprot.org/citations/17623778 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17623778 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17623778 | http://www.w3.org/2000/01/rdf-schema#comment | "G-protein-coupled receptors (GPCRs) transduce the binding of extracellular stimuli into intracellular signalling cascades that can lead to morphological changes. Here, we demonstrate that stimulation of the calcium-sensing receptor (CaSR), a GPCR that promotes chemotaxis by detecting increases in extracellular calcium, triggers plasma membrane (PM) ruffling via a pathway that involves beta-arrestin 1, Arf nucleotide binding site opener (ARNO), ADP-ribosylating factor 6 (ARF6) and engulfment and cell motility protein (ELMO). Expression of dominant negative beta-arrestin 1 or its knockdown with siRNA impaired the CaSR-induced PM ruffling response. Expression of a catalytically inactive ARNO also reduced CaSR-induced PM ruffling. Furthermore, beta-arrestin 1 co-immunoprecipitated with the CaSR and ARNO under resting conditions. Agonist treatment did not markedly alter beta-arrestin 1 binding to the CaSR or to ARNO but it did elicit the translocation and colocalisation of the CaSR, beta-arrestin 1 and ARNO to membrane protrusions. Furthermore, ARF6 and ELMO, two proteins known to couple ARNO to the cytoskeleton, were required for CaSR-dependent morphological changes and translocated to the PM ruffles. These data suggest that cells ruffle upon CaSR stimulation via a mechanism that involves translocation of beta-arrestin 1 pre-assembled with the CaSR or ARNO, and that ELMO plays an essential role in this CaSR-signalling-induced cytoskeletal reorganisation."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.03469"xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.03469"xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Martin S."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Martin S."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Bouschet T."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Bouschet T."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Henley J.M."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Henley J.M."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Kanamarlapudi V."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Kanamarlapudi V."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Mundell S."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/author | "Mundell S."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/pages | "2489-2497"xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/pages | "2489-2497"xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/title | "The calcium-sensing receptor changes cell shape via a beta-arrestin-1 ARNO ARF6 ELMO protein network."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/title | "The calcium-sensing receptor changes cell shape via a beta-arrestin-1 ARNO ARF6 ELMO protein network."xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/volume | "120"xsd:string |
| http://purl.uniprot.org/citations/17623778 | http://purl.uniprot.org/core/volume | "120"xsd:string |