| http://purl.uniprot.org/citations/17625228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17625228 | http://www.w3.org/2000/01/rdf-schema#comment | "Only 20-25% of families screened for BRCA1/2 mutations are found positive. Because only a positive result is informative, we studied the role of BRCA1/2 immunohistochemistry as an additional method for patient selection. From 53 high-risk-affected probands, 18 (34%) had available paraffin blocks of their tumors and were selected for this study. Mutation screening was done by conformation-sensitive gel electrophoresis and multiplex ligation-dependent probe amplification. For immunohistochemistry, 21 neoplastic specimens (15 breast carcinomas, 5 ovary neoplasms, and 1 rectal adenocarcinoma) were analyzed with BRCA1 (monoclonal antibody, Ab-1, oncogene) and BRCA2 (polyclonal antibody, Ab-2, oncogene) antibodies. Absence of the BRCA1 protein was confirmed in negative tumors by Western blotting. Seven patients were positive for BRCA1/2 mutations: 5 for BRCA1 and 2 for BRCA2. Four out of five positive patients had tumors negative for BRCA1 immunostaining, and the remaining 13 BRCA1-negative patients had positive BRCA1 immunostaining in all tumor samples. Sensitivity to predict for BRCA1 mutation carriers was 80%, and specificity was 100%, with a positive predictive value of 100% and a negative predictive value of 93%. This correlation was statistically significant (p=0.001). No correlation was observed for BRCA2. If larger studies confirm these results, high-risk patients with BRCA1-negative tumors should be screened first for this gene."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.org/dc/terms/identifier | "doi:10.1369/jhc.7a7209.2007"xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Andre S.P."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Brandao R.D."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Eugenio J.S."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Fernandes A.H."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Laranjeira C.T."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Machado P.M."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/author | "Vaz F.H."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/name | "J Histochem Cytochem"xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/pages | "1105-1113"xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/title | "Familial breast/ovarian cancer and BRCA1/2 genetic screening: the role of immunohistochemistry as an additional method in the selection of patients."xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://purl.uniprot.org/core/volume | "55"xsd:string |
| http://purl.uniprot.org/citations/17625228 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17625228 |
| http://purl.uniprot.org/citations/17625228 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17625228 |
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| http://purl.uniprot.org/uniprot/#_A0A068BDS6-mappedCitation-17625228 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17625228 |
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