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http://purl.uniprot.org/citations/17633465http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17633465http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the role of antiapoptotic Bcl-x(L) protein in megakaryocyte differentiation and maturation.

Methods

RNA interference was used to block the expression of Bcl-x(L) when K562 cells were induced to differentiate into megakaryocyte (CD61 + cells) by PDBu, and the expression of Bcl-x(L) was evaluated with flow cytometry and reverse transcription polymerase chain reaction (RT-PCR). The CD34 + cell fraction was positively isolated by using the MiniMACS system from normal bone marrow. Immunochemical staining and flow cytometry were used to detect the expression of Bcl-x(L) in the differentiation (CD41 + cells) of CD34 + cells induced by trombopoietin (TPO).

Results

Among K562 cells induced by PDBu, the percentage of CD6L + cells rapidly increased in 24 hours and maintained at a high positive level in 72 hours. When exposured to si-Bcl-x(L), the percentage of CD6 1 + cells only slightly increased in 72 hours. The expression of Bcl-x(L) mRNA was significantly decreased after transfection compared with that of control group, and Bcl-x(L) protein expression decreased correspondingly. After the CD34 + bone marrow cells having been treated with TPO for 5 days to 20 days, the Bcl-x(L)-megakaryocytes increased as the culture time prolonged, and there was a strong expression of Bcl-x(L) in immature megakaryocyte and an obviously decreased expression in degenerating megakaryocytes maturation.

Conclusions

Increased expression of antiapoptotic Bcl-x(L) may be essential to mature megakaryocyte. The down-regulation of antiapoptotic Bcl-x(L) in mature megakaryocyte may be crucial to platelets formation."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Liu B."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Ren H."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Han Z.B."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Han Z.C."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Lu S.H."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/author"Yang R.C."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/name"Zhongguo Yi Xue Ke Xue Yuan Xue Bao"xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/pages"374-378"xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/title"[Role of antiapoptotic Bcl-X(L) in megakaryocyte differentiation and maturation]."xsd:string
http://purl.uniprot.org/citations/17633465http://purl.uniprot.org/core/volume"29"xsd:string
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