| http://purl.uniprot.org/citations/17644514 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17644514 | http://www.w3.org/2000/01/rdf-schema#comment | "Microparticles from various cells are generated during inflammation. Platelet-derived microparticles (PMPs) harbor receptors that are not genuinely expressed by neutrophils. We tested whether or not functional glycoprotein IIb/IIIa (GPIIb/IIIa) receptors can be acquired by neutrophils via PMPs and whether these receptors participate in pro-inflammatory signaling. Surface expression was analyzed by flow cytometry and confocal microscopy. NF-kappaB activation was analyzed by Western blot experiments, electrophoretic mobility shift assays, and reverse transcription-PCR. Cell adhesion and spreading were estimated by myeloperoxidase assay and light microscopy. We found that PMPs transfer GPIIb/IIIa receptors to isolated and whole blood neutrophils via PMPs. We used specific antibodies in granulocyte macrophage colony-stimulating factor-treated neutrophils and observed that acquired GPIIb/IIIa receptors co-localized with beta2-integrins and cooperated in NF-kappaB activation. We show that Src and Syk non-receptor tyrosine kinases, as well as the actin cytoskeleton, control NF-kappaB activation. In contrast to NF-kappaB, acquisition of GPIIb/IIIa receptors was not necessary to induce adhesion to fibronectin or phosphatidylinositol 3-kinase/Akt signaling. When granulocyte macrophage colony-stimulating factor-stimulated neutrophils were incubated on fibronectin, strong NF-kappaB activation was observed, but only after loading with PMPs. Blocking either beta2-integrins or GPIIb/IIIa receptors abrogated this effect. Therapeutic GPIIb/IIIa inhibitors were similarly effective. The compounds also inhibited NF-kappaB-dependent tumor necrosis factor-alpha mRNA up-regulation. The data implicate GPIIb/IIIa receptors as new therapeutic targets in neutrophil-induced inflammation."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m704039200"xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/author | "Choi M."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/author | "Luft F.C."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/author | "Kettritz R."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/author | "Rolle S."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/author | "Wellner M."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/author | "Salanova B."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/pages | "27960-27969"xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/title | "Beta2-integrins and acquired glycoprotein IIb/IIIa (GPIIb/IIIa) receptors cooperate in NF-kappaB activation of human neutrophils."xsd:string |
| http://purl.uniprot.org/citations/17644514 | http://purl.uniprot.org/core/volume | "282"xsd:string |
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