| http://purl.uniprot.org/citations/17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17644519 | http://www.w3.org/2000/01/rdf-schema#comment | "Chemokine dimerization has been the subject of much interest in recent years as evidence has accumulated that different quaternary states of chemokines play different biological roles; the monomer is believed to be the receptor-binding unit, whereas the dimer has been implicated in binding cell surface glycosaminoglycans. However, although several studies have provided evidence for this paradigm by making monomeric chemokine variants or dimer-impaired chemokines, few have provided direct evidence of the receptor function of a chemokine dimer. We have produced a covalent dimer of the CC chemokine macrophage inflammatory protein-1beta (MIP-1beta) by placing a disulfide bond at the center of its dimer interface through a single amino acid substitution (MIP-1beta-A10C). This variant was shown to be a nondissociating dimer by SDS-PAGE and analytical ultracentrifugation. NMR reveals a structure largely the same as the wild type protein. In studies of glycosaminoglycan binding, MIP-1beta-A10C binds to a heparin-Sepharose column as tightly as the wild type protein and more tightly than monomeric variants. However, MIP-1beta-A10C neither binds nor activates the MIP-1beta receptor CCR5. It was found that the ability to activate CCR5 was recovered upon reduction of the intermolecular disulfide cross-link by incubation with 1 mm dithiothreitol. This work provides the first definitive evidence that the CC chemokine MIP-1beta dimer is not able to bind or activate its receptor and implicates the CC chemokine monomer as the sole receptor-interacting unit."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m702654200"xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/author | "Jin H."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/author | "Shen X."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/author | "Kong X."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/author | "LiWang P.J."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/author | "Baggett B.R."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/pages | "27976-27983"xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/title | "The human CC chemokine MIP-1beta dimer is not competent to bind to the CCR5 receptor."xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17644519 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17644519 |
| http://purl.uniprot.org/citations/17644519 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G6S9-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G7F7-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G7G0-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G7G7-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G7G9-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G3J8-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G3K0-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G3N4-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G3P0-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |
| http://purl.uniprot.org/uniprot/#_A0A089G3P4-mappedCitation-17644519 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17644519 |