| http://purl.uniprot.org/citations/17658618 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17658618 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17658618 | http://www.w3.org/2000/01/rdf-schema#comment | "'B-cell activating factor belonging to the TNF family' (BAFF) represents a cytokine produced by antigen presenting cells promoting B-cell maturation, activation and immunoglobulin class switching. In the present study, we demonstrate expression of BAFF on cultured monocyte-derived dendritic cells, which is further enhanced by interferon-alpha or interferon-gamma treatment. From these cells, porcine BAFF was cloned and the recombinant protein was expressed in mammalian cells with and without a FLAG tag at the carboxyl terminus. Only the protein without the FLAG tag was bioactive in vitro, and promoted B-cell survival and the differentiation of foot-and-mouth disease virus (FMDV)-specific memory B cells into antibody producing cells. Based on this result it was tested whether BAFF can enhance FMDV antibody responses in the context of a DNA vaccination. To this end, pigs were immunised with the anti-FMDV DNA vaccine plasmid pcDNA3.1/P1-2A3C3D and a pCI plasmid expressing porcine BAFF. Using a needle-free transdermal application method, also referred to as 'jet injection', pigs were vaccinated three times and their humoral response quantified by ELISA and a virus neutralisation test. After the third vaccination, three out of six animals vaccinated with the pcDNA3.1/P1-2A3C3D alone but none of the animals that also received the BAFF expressing plasmid had seroconverted. These data suggest that BAFF is not appropriate as a genetic adjuvant when applied as a simple co-injection with the antigen-encoding plasmid."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.vetimm.2007.06.021"xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.vetimm.2007.06.021"xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Summerfield A."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Summerfield A."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "McCullough K.C."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "McCullough K.C."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Neuhaus V."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Neuhaus V."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Bergamin F."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Bergamin F."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Saurer L."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/author | "Saurer L."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/name | "Vet. Immunol. Immunopathol."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/name | "Vet Immunol Immunopathol"xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/pages | "115-123"xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/pages | "115-123"xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/title | "Porcine B-cell activating factor promotes anti-FMDV antibodies in vitro but not in vivo after DNA vaccination of pigs."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/title | "Porcine B-cell activating factor promotes anti-FMDV antibodies in vitro but not in vivo after DNA vaccination of pigs."xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/volume | "120"xsd:string |
| http://purl.uniprot.org/citations/17658618 | http://purl.uniprot.org/core/volume | "120"xsd:string |