| http://purl.uniprot.org/citations/17662976 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17662976 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17662976 | http://www.w3.org/2000/01/rdf-schema#comment | "UNC-97/PINCH is an evolutionarily conserved protein that contains five LIM domains and is located at cell-extracellular matrix attachment sites known as cell adhesion complexes. To understand the role of UNC-97/PINCH in cell adhesion, we undertook a combined genetic and cell biological approach to identify the steps required to assemble cell adhesion complexes in Caenorhabditis elegans. First, we have generated a complete loss of function mutation in the unc-97 coding region. unc-97 null mutants arrest development during embryogenesis and reveal that the myofilament lattice and its attachment structures, which include PAT-4/ILK (integrin-linked kinase) and integrin fail to assemble into properly organized arrays. Although in the absence of UNC-97/PINCH, PAT-4/ILK and integrin fail to organize normally, they are capable of colocalizing together at the muscle cell membrane. Alternatively, in integrin and pat-4 mutants, UNC-97/PINCH fails to localize to the muscle cell membrane and instead is found diffusely throughout the muscle cell cytoplasm. In agreement with mammalian studies, we show that LIM domain 1 of UNC-97/PINCH is required for its interaction with PAT-4/ILK in yeast two-hybrid assays. Additionally, we find, by LIM domain deletion analysis, that LIM1 is required for the localization of UNC-97/PINCH to cell adhesion complexes. Our results provide evidence that UNC-97/PINCH is required for the development of C. elegans and is required for the formation of integrin based adhesion structures."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ydbio.2007.06.014"xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ydbio.2007.06.014"xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Norman K.R."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Norman K.R."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Qadota H."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Moerman D.G."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Moerman D.G."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Cordes S."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Cordes S."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Rahmani P."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/author | "Rahmani P."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/name | "Dev. Biol."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/name | "Dev. Biol."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/pages | "45-55"xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/pages | "45-55"xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/title | "UNC-97/PINCH is involved in the assembly of integrin cell adhesion complexes in Caenorhabditis elegans body wall muscle."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/title | "UNC-97/PINCH is involved in the assembly of integrin cell adhesion complexes in Caenorhabditis elegans body wall muscle."xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/volume | "309"xsd:string |
| http://purl.uniprot.org/citations/17662976 | http://purl.uniprot.org/core/volume | "309"xsd:string |