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| http://purl.uniprot.org/citations/17676993 | http://www.w3.org/2000/01/rdf-schema#comment | "Centromeres, the specialized chromatin structures that are responsible for equal segregation of chromosomes at mitosis, are epigenetically maintained by a centromere-specific histone H3 variant (CenH3). However, the mechanistic basis for centromere maintenance is unknown. We investigated biochemical properties of CenH3 nucleosomes from Drosophila melanogaster cells. Cross-linking of CenH3 nucleosomes identifies heterotypic tetramers containing one copy of CenH3, H2A, H2B, and H4 each. Interphase CenH3 particles display a stable association of approximately 120 DNA base pairs. Purified centromeric nucleosomal arrays have typical "beads-on-a-string" appearance by electron microscopy but appear to resist condensation under physiological conditions. Atomic force microscopy reveals that native CenH3-containing nucleosomes are only half as high as canonical octameric nucleosomes are, confirming that the tetrameric structure detected by cross-linking comprises the entire interphase nucleosome particle. This demonstration of stable half-nucleosomes in vivo provides a possible basis for the instability of centromeric nucleosomes that are deposited in euchromatic regions, which might help maintain centromere identity."xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pbio.0050218"xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/author | "Lindsay S."xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/author | "Henikoff S."xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/author | "Dalal Y."xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/name | "PLoS Biol"xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/pages | "e218"xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/title | "Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells."xsd:string |
| http://purl.uniprot.org/citations/17676993 | http://purl.uniprot.org/core/volume | "5"xsd:string |
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