| http://purl.uniprot.org/citations/17698844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17698844 | http://www.w3.org/2000/01/rdf-schema#comment | "Despite several investigations, the transcriptional mechanisms that regulate the expression of both type I collagen genes (COL1A1 and COL1A2) in either physiological or pathological situations, such as scleroderma, are not completely known. We have investigated the role of hc-Krox transcription factor on type I collagen expression by human dermal fibroblasts. hc-Krox exerted a stimulating effect on type I collagen protein synthesis and enhanced the corresponding mRNA steady-state levels of COL1A1 and COL1A2 in foreskin fibroblasts (FF), adult normal fibroblasts (ANF), and scleroderma fibroblasts (SF). Forced hc-Krox expression was found to up-regulate COL1A1 transcription through a -112/-61-bp sequence in FF, ANF, and SF. Knockdown of hc-Krox by short interfering RNA and decoy strategies confirmed the transactivating effect of hc-Krox and decreased substantially COL1A1 transcription levels in all fibro-blast types. The -112/-61-bp sequence bound specifically hc-Krox but also Sp1 and CBF. Attempts to elucidate the potential interactions between hc-Krox, Sp1, and Sp3 revealed that all of them co-immunoprecipitate from FF cellular extracts when a c-Krox antibody was used and bind to the COL1A1 promoter in chromatin immunoprecipitation assays. Moreover, hc-Krox DNA binding activity to its COL1A1-responsive element is increased in SF, cells producing higher amounts of type I collagen compared with ANF and FF. These data suggest that the regulation of COL1A1 gene transcription in human dermal fibroblasts involves a complex machinery that implicates at least three transcription proteins, hc-Krox, Sp1, and Sp3, which could act in concert to up-regulate COL1A1 transcriptional activity and provide evidence for a pro-fibrotic role of hc-Krox."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m705197200"xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Jimenez S.A."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Renard E."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Korn J."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Maquart F.X."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Galera P."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Kypriotou M."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Pujol J.P."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Chadjichristos C."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Widom R."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Beauchef G."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Oddos T."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/author | "Von Stetten O."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/pages | "32000-32014"xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/title | "Human collagen Krox up-regulates type I collagen expression in normal and scleroderma fibroblasts through interaction with Sp1 and Sp3 transcription factors."xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17698844 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17698844 |
| http://purl.uniprot.org/citations/17698844 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17698844 |
| http://purl.uniprot.org/uniprot/#_A8K6F4-mappedCitation-17698844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17698844 |
| http://purl.uniprot.org/uniprot/#_H9C5C5-mappedCitation-17698844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17698844 |
| http://purl.uniprot.org/uniprot/#_C4PGM0-mappedCitation-17698844 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17698844 |