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http://purl.uniprot.org/citations/17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17700029http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

Hepatitis C virus (HCV) F protein is a newly identified protein encoded by an alternative open reading frame that +1 overlaps core-encoding gene. It has been found that regulation of c-myc and p53 genes by HCV core protein is involved in liver cancer genesis. We wondered whether HCV F protein exerts similar or adverse regulatory effects on the transcription of c-myc and p53 genes.

Methods

HCV F gene-containing, plasmid pcDNA3.1-F and HCV core gene-containing pcDNA3.1-C were constructed and transiently transfected into HepG(2) cells. Real-time quantitative PCR or Western blotting was used to determine the changes at transcription or translation levels of c-myc and p53 genes.

Results

The transcription level of c-myc was much higher in pcDNA3.1-F transfected cells than those without plasmid transfected. Whereas the level of p53 transcription in pcDNA3.1-F transfected cells was lower than those in the parental cells. Moreover, levels of c-myc expression were up-regulated and those of p53 expression were down-regulated by HCV F protein.

Conclusions

HCV F protein is of regulatory properties in cellular oncogene c-myc and anti-oncogene p53, which may be implicated in the formation of hepatocellular carcinoma."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.org/dc/terms/identifier"doi:10.1159/000107271"xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Cao J."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Gao J."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Zhao L.J."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Zhu S.Y."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Luan J."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Qi Z.T."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Wu W.B."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/author"Shao S.W."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/name"Intervirology"xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/pages"341-346"xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/title"Hepatitis C virus F protein up-regulates c-myc and down-regulates p53 in human hepatoma HepG2 cells."xsd:string
http://purl.uniprot.org/citations/17700029http://purl.uniprot.org/core/volume"50"xsd:string
http://purl.uniprot.org/citations/17700029http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17700029
http://purl.uniprot.org/citations/17700029http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17700029
http://purl.uniprot.org/uniprot/#_A0A0B4J1R1-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
http://purl.uniprot.org/uniprot/#_A0A087WXZ1-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
http://purl.uniprot.org/uniprot/#_A0A087X1Q1-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
http://purl.uniprot.org/uniprot/#_A0A0C4KX50-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
http://purl.uniprot.org/uniprot/#_A0A0C4L134-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
http://purl.uniprot.org/uniprot/#_A0A218MJE5-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
http://purl.uniprot.org/uniprot/#_A0A087WT22-mappedCitation-17700029http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/17700029
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