| http://purl.uniprot.org/citations/17702749 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17702749 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17702749 | http://www.w3.org/2000/01/rdf-schema#comment | "The UT-A1 urea transporter mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. To transport urea, UT-A1 must be present in the plasma membrane. The purpose of this study was to screen for UT-A1-interacting proteins and to study the interactions of one of the identified potential binding partners with UT-A1. Using a yeast two-hybrid screen of a human kidney cDNA library with the UT-A1 intracellular loop (residues 409-594) as bait, we identified snapin, a ubiquitously expressed SNARE-associated protein, as a novel UT-A1 binding partner. Deletion analysis indicated that the C-terminal coiled-coil domain (H2) of snapin is required for UT-A1 interaction. Snapin binds to the intracellular loop of UT-A1 but not to the N- or C-terminal fragments. Glutathione S-transferase pulldown experiments and co-immunoprecipitation studies verified that snapin interacts with native UT-A1, SNAP23, and syntaxin-4 (t-SNARE partners), indicating that UT-A1 participates with the SNARE machinery in rat kidney inner medulla. Confocal microscopic analysis of immunofluorescent UT-A1 and snapin showed co-localization in both the cytoplasm and in the plasma membrane. When we co-injected UT-A1 with snapin cRNA in Xenopus oocytes, urea influx was significantly increased. In the absence of snapin, the influx was decreased when UT-A1 was combined with t-SNARE components syntaxin-4 and SNAP23. We conclude that UT-A1 may be linked to the SNARE machinery via snapin and that this interaction may be functionally and physiologically important for urea transport."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m705866200"xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m705866200"xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Chen G."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Chen G."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Rehm A."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Rehm A."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Mistry A.C."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Mistry A.C."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Froehlich O."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Froehlich O."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Klein J.D."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Klein J.D."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Sands J.M."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Sands J.M."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Mallick R."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/author | "Mallick R."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/pages | "30097-30106"xsd:string |
| http://purl.uniprot.org/citations/17702749 | http://purl.uniprot.org/core/pages | "30097-30106"xsd:string |