| http://purl.uniprot.org/citations/17703088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17703088 | http://www.w3.org/2000/01/rdf-schema#comment | "The transcription factor Ets2 and its transcriptional targets osteopontin (OPN) and osteocalcin (OC) are expressed in tissue-engineered bone constructs in vitro. Up to now little is known about the role of Ets2 in tissue-engineering applications. This study was intended to investigate the hypothesis that protein expression of Ets2 is correlated with the expression of bone-specific proteinsin tissue-engineeredbone constructs. Cell-seeded three-dimensional bone constructs manufactured with osteoblastic cells and poly(lactic-co-glycolic acid) polymer fleeces over a period of 21 days were analyzed by SDS-PAGE and Western blotting. The protein expression of OPN, OC, osteonectin and collagen type I was analyzed. Cellularity, alkaline phosphatase-specific activity and histology confirmed the osteoblastic phenotype of the constructs. Correlations between Ets2 expression and OPN and Ets2 and collagen type I expression could be detected during the phase of late osteoblastic differentiation between days 9 and 21. The correlation between OC and collagen type I was significant in this late stage of osteoblastic differentiation. These results suggest that there is a strong interplay of Ets2 with bone-specific proteins in cell-seeded three-dimensional bone constructs. This study is a crucial step to elucidate the complex interplay of bone-related proteins in the application of bone tissue engineering."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.org/dc/terms/identifier | "doi:10.1159/000107556"xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Stein E."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Turhani D."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Watzinger F."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Kneidinger D."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Smolik K."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Sutter W."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/author | "Wanschitz F."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/name | "Cells Tissues Organs"xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/pages | "213-220"xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/title | "Expression patterns of Ets2 protein correlate with bone-specific proteins in cell-seeded three-dimensional bone constructs."xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/17703088 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17703088 |
| http://purl.uniprot.org/citations/17703088 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17703088 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DG47-mappedCitation-17703088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17703088 |
| http://purl.uniprot.org/uniprot/#_P15036-mappedCitation-17703088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17703088 |
| http://purl.uniprot.org/uniprot/P15036 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17703088 |
| http://purl.uniprot.org/uniprot/A0A0C4DG47 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17703088 |