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http://purl.uniprot.org/citations/17767857http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17767857http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To compare binding activity of different zinc finger domain of human Kaiso with methylated CpG.

Methods

pGEX constructs with different human Kaiso domain were generated and then corresponding fusion proteins were induced and purified. Electrophoretic mobility shift assays were applied to evaluate the binding activity of fusion proteins with methylated CpG.

Results

The purified GST-KaisoZF fusion protein (without the POZ protein binding domain) could bind with methylated CpG probe specifically, but not for three or two zinc fingers without flanking domains.

Conclusion

Human zinc finger protein Kaiso could bind with methylated CpG specifically, only in the assistance of the neighboring flank sequence of the zinc finger domain."xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/author"Zhang B.Z."xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/author"Gu L.K."xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/author"Deng D.J."xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/name"Zhonghua Yu Fang Yi Xue Za Zhi 41"xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/pages"43-46"xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/title"[Methylation specific binding activity of zinc finger protein Kaiso]."xsd:string
http://purl.uniprot.org/citations/17767857http://purl.uniprot.org/core/volume"Suppl"xsd:string
http://purl.uniprot.org/citations/17767857http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17767857
http://purl.uniprot.org/citations/17767857http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17767857
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