| http://purl.uniprot.org/citations/1778989 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1778989 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1778989 | http://www.w3.org/2000/01/rdf-schema#comment | "Our recent work on the gene structures for human salivary (S-type) cystatins [Saitoh, E. et al. (1987) Gene 61, 329-338] has suggested that the structures of cystatins which we determined previously at the protein level lack N-terminal peptide portions of the full-sized intact forms. In the present study, attempts were made to isolate full-sized S-type cystatins by introducing methanol fractionation into the purification steps to suppress the enzymatic activity present in saliva. Full-sized cystatin SN and two phosphorylated forms of full-sized cystatin S were thus isolated. Analysis of one fraction indicated that this was a mixture of full-sized cystatin SA and non-phosphorylated cystatin S. The phosphorylation sites of cystatin S were determined to be Ser-Ser-Ser1(P)-Lys-Glu-Glu-for monophosphorylated cystatin S and Ser1(P)-Ser-Ser3(P)-Lys-Glu-Glu-for diphosphorylated cystatin S. Immunoblotting analysis with anti-cystatin S antiserum revealed that tears and seminal plasma also contained S-type cystatins, but diphosphorylated cystatin S was detected neither in tears nor in seminal plasma and no cystatin SN was found in seminal plasma. These data indicate that S-type cystatins are secreted into the oral cavity without significant degradation in salivary glands or ducts and that they are expressed tissue specifically."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123634"xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123634"xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Isemura S."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Isemura S."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Saitoh E."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Saitoh E."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Sanada K."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Sanada K."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Minakata K."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/author | "Minakata K."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/pages | "648-654"xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/pages | "648-654"xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/title | "Identification of full-sized forms of salivary (S-type) cystatins (cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms of cystatin S) in human whole saliva and determination of phosphorylation sites of cystatin S."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/title | "Identification of full-sized forms of salivary (S-type) cystatins (cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms of cystatin S) in human whole saliva and determination of phosphorylation sites of cystatin S."xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/volume | "110"xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://purl.uniprot.org/core/volume | "110"xsd:string |
| http://purl.uniprot.org/citations/1778989 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1778989 |
| http://purl.uniprot.org/citations/1778989 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1778989 |