| http://purl.uniprot.org/citations/17804403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17804403 | http://www.w3.org/2000/01/rdf-schema#comment | "Stabilization of spindle microtubules during anaphase is essential for proper chromosome segregation. Fin1 is a budding yeast protein that localizes to the poles and microtubules of the spindle during anaphase and contributes to spindle stability. The N-terminal half of Fin1 is phosphorylated at multiple sites by the cyclin-dependent kinase Clb5-Cdk1, and dephosphorylation in anaphase triggers its localization to the spindle. The C-terminal half of Fin1 contains coiled-coil motifs that are required for its self-association. Here we investigated the functional importance of the two regions of Fin1. Fin1 mutants lacking the C-terminal coiled-coil domains localized to spindle pole bodies but not along spindle microtubules. These mutants failed to self-associate and displayed reduced binding to microtubules in vitro but were functional in vivo and stabilized anaphase spindles when dephosphorylated. Deletion of the Fin1 C terminus suppressed the lethal phenotypes of the phospho-mutant Fin15A. Our findings suggest that the N-terminal region of Fin1 is sufficient for its regulated function as a spindle-stabilizing factor and that this function involves association with the spindle pole body. The ability of the C-terminal region to promote Fin1 self-association and microtubule binding may underlie the lethal effects of the deregulated Fin15A mutant."xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m705344200"xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/author | "Morgan D.O."xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/author | "Woodbury E.L."xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/pages | "32138-32143"xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/title | "The role of self-association in Fin1 function on the mitotic spindle."xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://purl.uniprot.org/core/volume | "282"xsd:string |
| http://purl.uniprot.org/citations/17804403 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17804403 |
| http://purl.uniprot.org/citations/17804403 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17804403 |
| http://purl.uniprot.org/uniprot/Q03898#attribution-CE8978E151A751044DF4C02B6BDC9FEC | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17804403 |
| http://purl.uniprot.org/uniprot/Q03898#attribution-F1D593A722C6B87EBEC823FD3F936C93 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/17804403 |
| http://purl.uniprot.org/uniprot/#_A0A8H8UN43-mappedCitation-17804403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17804403 |
| http://purl.uniprot.org/uniprot/#_Q03898-mappedCitation-17804403 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17804403 |
| http://purl.uniprot.org/uniprot/A0A8H8UN43 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17804403 |
| http://purl.uniprot.org/uniprot/Q03898 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17804403 |