http://purl.uniprot.org/citations/17846325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/17846325 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundEmbryonic stem (ES) cells are distinguished by their capacity for self-renewal and pluripotency. Here we characterize the differentiation of ES cell-derived endothelial cells (ESC-ECs), use molecular imaging techniques to examine their survival in vivo, and determine the therapeutic efficacy of ESC-ECs for restoration of cardiac function after ischemic injury.Methods and resultsMurine ES cells were transfected with a construct composed of a vascular endothelial cadherin promoter driving enhanced green fluorescence protein (pVE-cadherin-eGFP). Differentiation of ES cells to ECs was detected by FACS analysis using Flk-1 (early EC marker at day 4) and VE-cadherin (late EC marker at day 8). After isolation, these ESC-ECs express endothelial cell markers similar to adult mouse lung endothelial cells, form vascular-like channels, and incorporate DiI-labeled acetylated low-density lipoprotein (DiI-Ac-LDL). For in vivo imaging, ES cells were transduced with an ubiquitin promoter driving firefly luciferase and monomeric red fluorescence protein (pUb-Fluc-mRFP). A robust correlation exists between Fluc signals and cell numbers by ex vivo imaging analysis (R2=0.98) and by in vitro enzyme assay (R2=0.94). Afterward, 5x10(5) ESC-ECs or PBS (as control) was injected into the hearts of mice undergoing LAD ligation (n=15 per group). Bioluminescence imaging showed longitudinal survival of transplanted ESC-ECs for approximately 8 weeks. Echocardiogram demonstrated significant functional improvement in the ESC-EC group compared with control (P=0.04). Finally, postmortem analysis confirmed increased presence of small capillaries and venules in the infarcted zones by CD31 staining.ConclusionsThis is the first study to track the fate and function of transplanted ESC-ECs in the heart. With further validation, these ESC-ECs could become a valuable source of cell therapy for induction of angiogenesis in the treatment of myocardial ischemia."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.org/dc/terms/identifier | "doi:10.1161/circulationaha.106.680561"xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Li Z."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Xie X."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Patel M."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Cao F."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Wu J.C."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Kraft D."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Gambhir S.S."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Cooke J.P."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Robbins R.C."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Sheikh A.Y."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/author | "Wu J.C.'"xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/name | "Circulation"xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/pages | "I46-54"xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/title | "Differentiation, survival, and function of embryonic stem cell derived endothelial cells for ischemic heart disease."xsd:string |
http://purl.uniprot.org/citations/17846325 | http://purl.uniprot.org/core/volume | "116"xsd:string |
http://purl.uniprot.org/citations/17846325 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17846325 |
http://purl.uniprot.org/citations/17846325 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17846325 |
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http://purl.uniprot.org/uniprot/#_P55284-mappedCitation-17846325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17846325 |
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http://purl.uniprot.org/uniprot/Q3TJ23 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17846325 |