| http://purl.uniprot.org/citations/17855557 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17855557 | http://www.w3.org/2000/01/rdf-schema#comment | "MicroRNAs (miRNAs) are a class of 19-to 23-nt, small, noncoding RNAs, which bind the 3' UTR of target mRNAs to mediate translational repression in animals. miRNAs have been shown to regulate developmental processes, such as self-renewal of stem cells, neuronal differentiation, myogenesis, and cancer. A functional role of miRNAs in the regulation of neurotransmitter synthesis has yet to be ascribed. We used mesenchymal stem cells (MSCs) as a model to study miRNA-mediated neurotransmitter regulation in developing neuronal cells. MSCs are mesoderm-derived cells, primarily resident in adult bone marrow, which can generate functional neuronal cells. We have previously shown that human MSC-derived neuronal cells express the neurotransmitter gene, Tac1, but do not synthesize the gene's encoded peptide, the neurotransmitter substance P (SP), unless stimulated with the inflammatory mediator IL-1alpha. These findings suggested a potential role for miRNAs in the regulation of SP synthesis. Here, we report on the miRNA profile of undifferentiated human MSCs and MSC-derived neuronal cells by using miRNA-specific bioarrays. miRNAs that were increased in the neuronal cells and decreased after IL-1alpha stimulation were analyzed by the miRanda algorithm to predict Tac1 mRNA targets. Putative miR-130a, miR-206, and miR-302a binding sites were predicted within the 3' UTR of Tac1. Target validation using a luciferase reporter system confirmed the miR-130a and miR-206 sites. Specific inhibition of miR-130a and miR-206 in the neuronal cells resulted in SP synthesis and release. The studies provide a different approach in ascribing a new regulatory role for miRNAs in regulating neurotransmitter synthesis."xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0703037104"xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/author | "Rameshwar P."xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/author | "Greco S.J."xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/pages | "15484-15489"xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/title | "MicroRNAs regulate synthesis of the neurotransmitter substance P in human mesenchymal stem cell-derived neuronal cells."xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://purl.uniprot.org/core/volume | "104"xsd:string |
| http://purl.uniprot.org/citations/17855557 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17855557 |
| http://purl.uniprot.org/citations/17855557 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17855557 |
| http://purl.uniprot.org/uniprot/#_P20366-mappedCitation-17855557 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17855557 |
| http://purl.uniprot.org/uniprot/#_Q9Y494-mappedCitation-17855557 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17855557 |
| http://purl.uniprot.org/uniprot/Q9Y494 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17855557 |
| http://purl.uniprot.org/uniprot/P20366 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17855557 |