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http://purl.uniprot.org/citations/17881917http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/17881917http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

To review the role of promyelocytic leukemia zinc finger (PLZF), a transcriptional repressor and negative regulator of cell cycling, in the proliferation of cultured human corneal endothelial cells (HCECs).

Methods

The expression pattern of PLZF mRNA was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time quantitative PCR in HCECs and normal human corneal epithelia. The effect of cell-cell contact on expression of the PLZF gene was studied after incubation of the cultured HCECs in EDTA. The proliferation rate of cultured HCECs was assayed by a real-time electronic sensing (RT-CES) system, and DNA microarray analysis was performed to find the PLZF-regulating genes in cultured HCECs infected with LacZ- and PLZF-carrying adenoviruses (Ad-LacZ, Ad-PLZF).

Results

PLZF mRNA was expressed in HCECs in vivo and in completely confluent HCECs but not in subconfluent HCECs in vitro. Real-time PCR showed that the expression of PLZF mRNA was decreased by approximately 20-fold when incubated with EDTA and returned to a normal level as the cell-cell contact reformed. Cell proliferation assay by the RT-CES system showed that infection of cultured HCECs with Ad-PLZF inhibited proliferation.

Conclusions

These findings suggest that PLZF plays an important role in the suppression of proliferation of HCECs."xsd:string
http://purl.uniprot.org/citations/17881917http://purl.org/dc/terms/identifier"doi:10.1097/ico.0b013e31812f6b67"xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/author"Ohashi Y."xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/author"Higashiyama S."xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/author"Shiraishi A."xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/author"Joko T."xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/date"2007"xsd:gYear
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/name"Cornea"xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/pages"S55-8"xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/title"Role of promyelocytic leukemia zinc finger protein in proliferation of cultured human corneal endothelial cells."xsd:string
http://purl.uniprot.org/citations/17881917http://purl.uniprot.org/core/volume"26"xsd:string
http://purl.uniprot.org/citations/17881917http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/17881917
http://purl.uniprot.org/citations/17881917http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/17881917
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http://purl.uniprot.org/uniprot/Q05516http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/17881917
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