| http://purl.uniprot.org/citations/17901360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17901360 | http://www.w3.org/2000/01/rdf-schema#comment | "Smooth muscle expresses the Ialpha and the Ibeta isoforms of cGMP-dependent protein kinase I (cGKI). Inactivation of the murine cGKI gene prkg1 leads to multiple phenotypes and premature death at approximately 6 weeks. We reconstituted mice with the cGKIalpha or -Ibeta isozyme to test which isozyme was needed to support basic smooth muscle functions. Mice were generated by gene targeting. The cGKIalpha or the -Ibeta coding sequences were placed under the control of the SM22alpha promoter to express either isoform selectively in smooth muscle cells (SM-Ialpha or SM-Ibeta transgene). To generate smooth muscle-specific cGKIalpha or cGKIbeta rescue mice, the SM-Ialpha or SM-Ibeta transgenes were crossed on a cGKI-/-genetic background. The levels of cGKIalpha or -Ibeta expression were comparable to endogenous cGKI expression in wild-type aortic and intestinal smooth muscles. In cGKIalpha or -Ibeta rescue mice, expression of the isozymes was not detectable in non-smooth muscle tissues and cells. Median survival time of the Ialpha and Ibeta rescue mice was 52 weeks. Both isozymes mediated the 8-bromo-cGMP-induced relaxation of precontracted jejunum and aorta muscle strips. Activation of both isozymes reduced hormone- or K+-induced [Ca2+]i levels. The cGKIalpha and cGKIbeta rescue mice did not show a significant difference in intestinal passage time of BaSO4 in comparison with wild-type animals. Telemetric blood pressure measurements in conscious freely moving animals did not show differences between rescues and control mice in basal blood pressure and its regulation by DETA-NO, sodium nitroprusside, carbachol, or Y-27632. These results show that cGKI in smooth muscle is essential and that either cGKI isozyme alone can rescue basic vascular and intestinal smooth muscle functions."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.org/dc/terms/identifier | "doi:10.1161/circresaha.107.154351"xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Feil R."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Hofmann F."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Bernhard D."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Weber S."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Valtcheva N."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Feil S."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Wegener J.W."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Schlossmann J."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Weinmeister P."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Lukowski R."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/author | "Worner R."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/name | "Circ Res"xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/pages | "1096-1103"xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/title | "Rescue of cGMP kinase I knockout mice by smooth muscle specific expression of either isozyme."xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://purl.uniprot.org/core/volume | "101"xsd:string |
| http://purl.uniprot.org/citations/17901360 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17901360 |
| http://purl.uniprot.org/citations/17901360 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17901360 |
| http://purl.uniprot.org/uniprot/#_A0A1L1STN8-mappedCitation-17901360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17901360 |
| http://purl.uniprot.org/uniprot/#_P0C605-mappedCitation-17901360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17901360 |
| http://purl.uniprot.org/uniprot/#_P70460-mappedCitation-17901360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17901360 |
| http://purl.uniprot.org/uniprot/#_A0A5F8MPU4-mappedCitation-17901360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17901360 |