| http://purl.uniprot.org/citations/17913870 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/17913870 | http://www.w3.org/2000/01/rdf-schema#comment | "The Na+/H+ exchanger (NHE) has a key role in intracellular pH ([pH]i) regulation of the syncytiotrophoblast in the human placenta and may have a role in the life cycle of this cell. In other cells the NHE (actually a family of up to 9 isoforms) is regulated by a variety of factors, but its regulation in the syncytiotrophoblast has not been studied. Here, we tested the hypotheses that EGF and sphingosine-1-phosphate (S1P), both of which affect trophoblast apoptosis and, in other cell types, NHE activity, stimulate syncytiotrophoblast NHE activity. Villous fragments from term human placentas were loaded with the pH-sensitive dye, BCECF. NHE activity was measured by following the recovery of syncytiotrophoblast [pH]i following an imposed acid load, in the presence and absence of EGF, S1P, and specific inhibitors of NHE activity. Both EGF and S1P caused a dose-dependent upregulation of NHE activity in the syncytiotrophoblast. These effects were blocked by amiloride 500 microM (a nonspecific NHE blocker) and HOE694 100 microM (NHE blocker with NHE1 and 2 isoform selectivity). Effects of EGF were also reduced by the NHE3 selective blocker S3226 (used at 1 microM). These data provide the first evidence that both EGF and S1P stimulate NHE activity in the syncytiotrophoblast; they appear to do so predominantly by activating the NHE1 isoform."xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpregu.00328.2007"xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/author | "Sibley C.P."xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/author | "Speake P.F."xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/author | "Johnstone E.D."xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/date | "2007"xsd:gYear |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/name | "Am J Physiol Regul Integr Comp Physiol"xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/pages | "R2290-4"xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/title | "Epidermal growth factor and sphingosine-1-phosphate stimulate Na+/H+ exchanger activity in the human placental syncytiotrophoblast."xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://purl.uniprot.org/core/volume | "293"xsd:string |
| http://purl.uniprot.org/citations/17913870 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/17913870 |
| http://purl.uniprot.org/citations/17913870 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/17913870 |
| http://purl.uniprot.org/uniprot/#_B4DTZ6-mappedCitation-17913870 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17913870 |
| http://purl.uniprot.org/uniprot/#_B2RAH2-mappedCitation-17913870 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17913870 |
| http://purl.uniprot.org/uniprot/#_P19634-mappedCitation-17913870 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/17913870 |
| http://purl.uniprot.org/uniprot/B2RAH2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17913870 |
| http://purl.uniprot.org/uniprot/P19634 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17913870 |
| http://purl.uniprot.org/uniprot/B4DTZ6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/17913870 |